HT1080 Cell Lysate (AHL013)

Cell and tissue lysates come lyophilized for maximum stability and utility. Lyophilized lysates can be stored at -80C or -20C for up to 10 years without functional compromise. Simply reconstitute to desired concentration with sterile, distilled water or 1XPBS, adding glycerol to a final concentration of 10%. After the addition of sterile, distilled water or PBS, recap vial and briefly vortex making sure that the liquid has been evenly distributed and has covered the entire inside of the vial so all lyophilized lysate goes into solution. After vortexing, centrifuge the solution again. Aviva recommends storing small, single-use aliquots of reconstituted lysates to minimize freeze-thaw cycles.
Heat reconstituted cell/tissue lysate at 100C for 10min, cool down and load on to gel. The lysates come lyophilized with SDS-PAGE buffer containing the reducing agent, DTT.