Manufacturer |
AVIVA Systems Biology
|
Category |
|
Type |
Antibody Polyclonal |
Specific against |
Human, Mouse, Rat, Rabbit, Guinea Pig, Bovine, Canine, Equine |
Format |
Lyophilized powder |
Applications |
WB |
Amount |
100ul |
Host |
Rabbit |
Item no. |
ARP48725_P050 |
eClass 6.1 |
32160702 |
eClass 9.0 |
32160702 |
Available |
|
Alias |
RPC1, RPC155, hRPC155, ADDH, HLD7 |
Gene symbol |
POLR3A |
Protein name |
DNA-directed RNA polymerase III subunit RPC1 |
Protein size |
1390 |
Molecular weight |
156kDa |
Subunit |
RPC1 |
Product format |
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Gene id |
11128 |
Reconstitution and storage |
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Partner proteins |
SUMO2. UBC. ASB13. NEDD4. POLR3H. VPS18. POLR3B. POLR1D. CRCP. POLR1A. POLR3C. POLR1C. SMARCA5. POLR2H. POLR2E. POLR3D. IKBKG. PCBD1. EWSR1. RPAP2. RPAP3. RPAP1. GPN1. RAE1. POLR3E. PKP2. GTF2B. |
Description of target |
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition. |
Homology |
Cow: 79%; Dog: 86%; Guinea Pig: 86%; Horse: 79%; Human: 100%; Mouse: 79%; Rabbit: 86%; Rat: 86%; Yeast: 85% |
Purification |
Affinity Purified |
Clonality |
Polyclonal |
Immunogen |
The immunogen is a synthetic peptide directed towards the middle region of human POLR3A |
Drywet |
Wet Ice |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.