Item no. |
OAPC00120 |
Manufacturer |
AVIVA Systems Biology
|
Amount |
100 ul |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid. 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots. |
Applications |
WB |
Specific against |
Human, Mouse, Rat, Fish, Bovine, Canine |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Available |
|
Description |
Specific for the ca.51k alpha 2-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 2-subunit knockout animals. Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled. |
Short description |
To order by phone call us at (888) 880-0001, fax us at (858) 552-6975 or send an email to mailto:infohoelzel.de'>infohoelzel.de. Aviva manufactures this antibody so we can offer the best price. Please contact us to request pricing information.All of Aviva&rsquo, s products are guaranteed for the applications and experimental sample types mentioned in the datasheet below. Are you curious if this product will work for you? Please contact us at mailto:infohoelzel.de'>infohoelzel.de |
Gene symbol |
GABRA2 |
Molecular weight |
ca.51kDa |
Gene_id |
61856 |
Reconstitution and storage |
For long term storage -20C is recommended. Stable at -20C for at least 1 year. |
Description of target |
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alpha s, four beta s and four gamma s, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990, Whiting et al., 1999, Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha - and beta -subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma -subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha -subunits of the receptor (McKernan et al., 2000, Mehta and Ticku, 1998, Ogris et al., 2004, Pöltl et al., 2003). |
Specificity |
Specific for the ca.51k alpha 2-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha 2-subunit knockout animals. |
Homology short |
Rat, Mouse, Human, Bovine, Non-human primate, Zebra fish, Canine |
Purification |
Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled. |
Immunogen |
Fusion protein from the cytoplasmic loop of the alpha 2-subunit of rat GABAA receptor. |
Application Info |
The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with bovine, canine, human, non-human primate and Zebrafish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.Western blot of mouse brain lysates from wild type (Control) and alpha 2-knockout (alpha 2-K/O) animals showing specific immunolabeling of the ca.51k alpha 2-subunit of the GABAA-R. The labeling was absent from a lysate prepared from alpha 2-knockout animals. |
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