Item no. |
P-1010 |
Manufacturer |
Aves Labs
|
Amount |
2 mL |
Category |
|
Type |
Reagents |
Format |
Affinity Purified |
Applications |
IP |
Specific against |
Chicken (Gallus gallus domesticus) |
Host |
Caprine |
Isotype |
IgG |
Conjugate/Tag |
Agarose |
ECLASS 10.1 |
42051003 |
ECLASS 11.0 |
42051003 |
UNSPSC |
12000000 |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Type |
Immunoprecipitation Reagent |
Manufacturer - Targets |
Chicken IgY |
Country of Origin |
United States |
Shipping Temperature |
Cold Pack |
Storage Conditions |
Store at 4°C in the dark. Under these conditions, this product should have a shelf life of at least twelve months, provided they remain sterile. |
Shipping Method |
Overnight Priority |
Buffer |
Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent) |
Quality Control |
The capacity of PrecipHen® was determined by measuring the 2.5 mLs of PrecipHen. One milliliter aliquots of chicken IgY (200 µg/mL in TE buffer) were applied to the column and fractions were collected. A similar column of agarose (non-NHS activated) was used for comprison. The concentration of IgY passing through each column was measured, and the total amount of IgY that bound to the column was calculated. |
Description |
Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with chicken antibodies in immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and protein purification applications. |
Manufacturer - Statement |
Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with IP, CHiP, and protein purification applications. |
Antibody Registry ID (RRID) |
AB_2313533 |
Clonality |
Polyclonal |
Manufacturer - Specificity |
H&L; does not cross-react with mammalian IgGs. |
Manufacturer - Form |
Affinity Purified |
Physical State |
Agarose beads in buffer, binding capacity 1.0 mg of chicken IgY per ml bed volume
1.0 mg of chicken IgY per ml bed volume |
Expiration |
12 months from receipt of product |
Usage Statement |
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans. |
Production Notes |
Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”). Sodium azide was then added to a final concentration of 0.02% (w/v). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.