Comparison

PrecipHen® Immunoprecipitation Reagent

Item no. P-1010
Manufacturer Aves Labs
Amount 2 mL
Category
Type Reagents
Format Affinity Purified
Applications IP
Specific against Chicken (Gallus gallus domesticus)
Host Caprine
Isotype IgG
Conjugate/Tag Agarose
ECLASS 10.1 42051003
ECLASS 11.0 42051003
UNSPSC 12000000
Shipping condition Cool pack
Available
Manufacturer - Type
Immunoprecipitation Reagent
Manufacturer - Targets
Chicken IgY
Country of Origin
United States
Shipping Temperature
Cold Pack
Storage Conditions
Store at 4°C in the dark. Under these conditions, this product should have a shelf life of at least twelve months, provided they remain sterile.
Shipping Method
Overnight Priority
Buffer
Phosphate-buffered saline (PBS) (pH 7.2), sodium azide (0.02%, w/v) (as an anti-microbial agent)
Quality Control
The capacity of PrecipHen® was determined by measuring the 2.5 mLs of PrecipHen. One milliliter aliquots of chicken IgY (200 µg/mL in TE buffer) were applied to the column and fractions were collected. A similar column of agarose (non-NHS activated) was used for comprison. The concentration of IgY passing through each column was measured, and the total amount of IgY that bound to the column was calculated.
Description
Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with chicken antibodies in immunoprecipitation (IP), chromatin immunoprecipitation (ChIP), and protein purification applications.
Manufacturer - Statement
Our PrecipHen® is made from goat anti-chicken IgY antibodies covalently attached to agarose beads. It is designed for use with IP, CHiP, and protein purification applications.
Antibody Registry ID (RRID)
AB_2313533
Clonality
Polyclonal
Manufacturer - Specificity
H&L; does not cross-react with mammalian IgGs.
Manufacturer - Form
Affinity Purified
Physical State
Agarose beads in buffer, binding capacity 1.0 mg of chicken IgY per ml bed volume



















1.0 mg of chicken IgY
per ml bed volume
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Immune sera was collected from goats that were hyper-immunized with purified chicken IgY. Goat anti-chicken IgY antibodies were affinity purified from the sera over a chicken IgY column, and then conjugated to N-hydroxysuccinimide (NHS)-activated agarose beads. After blocking residual NHS sites, the agarose beads were washed exhaustively with 50 mM HEPES buffer (pH 7.6) with 5 mM EDTA (“TE buffer”). Sodium azide was then added to a final concentration of 0.02% (w/v).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 2 mL
Available: In stock
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