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Anti-Green Fluorescent Protein (GFP) Antibody

Anti-Green Fluorescent Protein (GFP) Antibody

Item no.	GFP-1010
Manufacturer	Aves Labs
Amount	100 uL
Quantity options	100 uL (100 ul) x 10 400 uL (400 ul) x 10
Category	Antibodies Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB, IHC, ICC, ELISA
Specific against	other
Host	Chicken
Isotype	IgY
Conjugate/Tag	Unconjugated
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Type	Primary Antibody
Manufacturer - Category	Anti-Tag Antibodies
Manufacturer - Targets	Green Fluorescent Protein (GFP)
Country of Origin	United States
Storage Conditions	Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.
Molecular Weight	27 kDa
Concentration	10 mg/mL
Shipping Method	Overnight Priority
Buffer	Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.
Product Info	Green Fluorescent Protein (GFP) Antibody. Chickens were immunized with recombinant GFP protein. After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using an agarose matrix to which the recombinant protein was attached. The final preparation of this product involved mixing affinity purified anti-GFP antibody (final concentration of 25 ug/ml) with IgY fractions of anti-GFP antibody (final concentration of 10 mg/ml) in PBS with 50% glycerol. This preparation was then filter-sterilized.
Quality Control	Antibodies were analyzed by western blot analysis (1:5000 dilution) and immunohistochemistry (1:500 dilution) using transgenic mice expressing the GFP gene product. Western blots were performed using BlokHen® (Aves Labs) as the blocking reagent, and HRP-labeled goat anti-chicken antibodies (Aves Labs, Cat. #H-1004) as the detection reagent. Immunohistochemistry used tetramethyl rhodamine-labeled anti-chicken IgY.
Description	Our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects n/a Green Fluorescent Protein (GFP), and is mixture of IgY fraction and affinity-purified antibodies. It is great for use in ELISA, IHC, ICC, WB.
Manufacturer - Statement	We produce our Anti-Green Fluorescent Protein (GFP) chicken polyclonal primary antibody. It is great in ELISA, IHC, ICC, WB and is mixture of IgY fraction and affinity-purified antibodies.
Target Description	Green Fluorescent Protein (GFP) is a naturally fluorescent protein originally derived from jellyfish. GFP has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors which have become useful and ubiquitous tools in transgenic experiments. Fluorescent proteins enable a wide range of applications where they have functioned as cell lineage tracers, reporters of gene expression, or as a measure of protein-protein interactions.
Antibody Registry ID (RRID)	AB_2307313
Clonality	Polyclonal
Dilution Range ICC	1:2000-1:5000
Dilution Range IHC	1:2000-1:5000
Dilution Range Western Blot	1:5000-1:10000
Immunogen	Recombinant GFP expressed in Escherichia coli
Manufacturer - Form	Mixture of IgY fraction and affinity-purified antibodies
Expiration	12 months from receipt of product
Trial Size	Yes
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes	Chickens were immunized with recombinant green fluorescent protein (GFP) emulsified in Freund’s adjuvant. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using GFP conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (30 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with sodium azide and then filter-sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 uL

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Delivery expected until 3/28/2025

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