Comparison

Anti-HA Epitope Tag Antibody

Item no. ET-HA100
Manufacturer Aves Labs
Amount 1 mL
Category
Type Antibody
Format Liquid
Applications WB, IP, IHC, ICC, ELISA
Specific against other
Host Chicken
Isotype IgY
Conjugate/Tag Unconjugated
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HA, HA Tag, HA Epitope Tag, Hemagglutinin
Shipping condition Cool pack
Available
Manufacturer - Type
Primary Antibody
Manufacturer - Category
Anti-Tag Antibodies
Manufacturer - Targets
HA Epitope Tag Antibody
Country of Origin
United States
Storage Conditions
Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.
Concentration
100 µg/mL
Shipping Method
Overnight Priority
Buffer
Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS, " pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Product Info
Anti-HA (influenza hemagglutinin) polyclonal antibodies were generated in chickens against a synthetic peptide containing the influenza hemaglutinin epitope (YPYDVPDYA) conjugated to keyhole limpet hemocyanin (KLH). This is the same epitope as recognized by the HA.11 mouse monoclonal antibody. Eggs were collected from hyperimmunized hens, and the IgY fraction was isolated using a proprietary method. Epitope-specific antibodies were affinity purified over a peptide column, dialyzed against PBS, and the concentration was adjusted to 0.1 mg/ml. Sodium azide was added to a concentration of 0.02% (w/v), and 1.0 ml aliquots prepared.
Quality Control
Antibodies were analyzed using ELISA and western blots using an N-terminal HA-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.
Description
Our Anti-HA Epitope Tag Antibody chicken polyclonal primary antibody from Aves Labs is produced in-house. It detects HA Epitope Tag Antibody, and is affinity-purified IgY. It is great for use in ELISA, IHC, ICC, IP, WB.
Manufacturer - Statement
We produce our Anti-HA Epitope Tag Antibody chicken polyclonal primary antibody. It is great in ELISA, IHC, ICC, IP, WB and is affinity-purified IgY.
Target Description
The synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA).
Antibody Registry ID (RRID)
AB_2313511
Clonality
Polyclonal
Dilution Range ICC
1:500
Dilution Range IHC
1:500
Dilution Range Western Blot
1:5000
Immunogen
Synthetic peptide
Manufacturer - Form
Affinity-Purified IgY
Expiration
12 months from receipt of product
Usage Statement
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes
Chickens were immunized with a synthetic peptide containing the influenza hemagglutinin (HA) epitope (i.e., YPYDVPDYA) conjugated to keyhole limpet hemocyanin (KLH). After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity purified using a peptide column. The concentration of the eluate was then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 1 mL
Available: In stock
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