Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody

Anti-Tag Antibodies

United States

117 kDa

Overnight Priority

Beta-Galactosidase (lacZ) Antibody. Chickens were immunized with purified b-galactosidase protein (lac Z from E. coli). After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using an agarose matrix to which the whole protein was attached. The final preparation of this product involved mixing affinity purified anti-beta-galactosidase antibody (final concentration of 25 ug/ml) with IgY fractions of anti-beta-galactosidase antibody (final concentration of 10 mg/ml) in PBS with 50% glycerol. This preparation was then filter-sterilized.

Our Beta-Galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity. It even works well with fusion proteins of LacZ!

Beta Galactosidase is the product of the LacZ gene and is widely used as a reporter marker to monitor gene expression.

Polyclonal

1:2500-1:5000

1:5000-1:10000

E Coli

12 months from receipt of product

Chickens were immunized with purified ß-Galactosidase (LacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with thimerosal and then filter-sterilized.