« Back
Home /
Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody

Anti-Beta-Gal (Beta-Galactosidase, LacZ) Antibody

Item no.	BGL-1010
Manufacturer	Aves Labs
Amount	100 uL
Quantity options	100 uL 400 uL
Category	Antibodies Immunohistochemistry Western Blot Immunocytochemistry Primary Antibodies
Type	Antibody
Format	Liquid
Applications	WB, IHC, ICC
Specific against	other
Host	Chicken
Isotype	IgY
Conjugate/Tag	Unconjugated
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Beta-Gal, Beta-Galactosidase, LacZ
Shipping condition	Cool pack
Available
Manufacturer - Type	Primary Antibody
Manufacturer - Category	Anti-Tag Antibodies
Manufacturer - Targets	Beta-Gal (Beta-Galactosidase, LacZ)
Country of Origin	United States
Storage Conditions	Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile.
Molecular Weight	117 kDa
Concentration	10 mg/mL
Shipping Method	Overnight Priority
Buffer	Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with thimerosal (0.01%, w/v) as an anti-microbial agent.
Product Info	Beta-Galactosidase (lacZ) Antibody. Chickens were immunized with purified b-galactosidase protein (lac Z from E. coli). After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using an agarose matrix to which the whole protein was attached. The final preparation of this product involved mixing affinity purified anti-beta-galactosidase antibody (final concentration of 25 ug/ml) with IgY fractions of anti-beta-galactosidase antibody (final concentration of 10 mg/ml) in PBS with 50% glycerol. This preparation was then filter-sterilized.
Quality Control	This antibody preparation was analyzed by immunohistochemistry (at a dilution of 1:5000) using tissues infected with a virus bearing the LacZ gene. Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1:2000 dilution, Aves Labs Cat.# F-1005).
Description	Our Beta-Galactosidase antibody recognizes the LacZ gene product with high titre and high specificity. Since this antibody was generated in chickens, it can be used together with your existing rabbit and mouse primary antibodies for double immunostaining with no secondary antibody cross reactivity. It even works well with fusion proteins of LacZ!
Manufacturer - Statement	We produce our Anti-Beta-Gal (Beta-Galactosidase, LacZ) chicken polyclonal primary antibody. It is great in IHC, ICC, WB and is mixture of IgY fraction and affinity-purified antibodies.
Target Description	Beta Galactosidase is the product of the LacZ gene and is widely used as a reporter marker to monitor gene expression.
Antibody Registry ID (RRID)	AB_2313508
Clonality	Polyclonal
Dilution Range ICC	1:2500-1:5000
Dilution Range IHC	1:2500-1:5000
Dilution Range Western Blot	1:5000-1:10000
Dilution Range Western Blot Brain	1:5000-1:10000
Immunogen	Native protein expressed Escherichia coli
Immunogen Species	E Coli
Manufacturer - Form	Mixture of IgY fraction and affinity-purified antibodies
Expiration	12 months from receipt of product
Trial Size	Yes
Usage Statement	These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
Production Notes	Chickens were immunized with purified ß-Galactosidase (LacZ) protein emulsified in Freund’s adjuvants. After multiple injections, eggs were collected from the hens, IgY fractions were prepared from the yolks, and then affinity-purified antibodies were prepared using ß-Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/mL) and purified IgY (10 mg/mL), mixed with glycerol 1:1 (v/v) (to prevent freezing at -20°C), augmented with thimerosal and then filter-sterilized.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BGL-1010-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 100 uL

available

Delivery expected until 3/28/2025

Express Delivery: Save 8 days

Compare

Add to wishlist

Recommend by mail