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EnzyChrom™ Lactose Assay Kit

EnzyChrom™ Lactose Assay Kit

Item no.	BAS-ELAC-100
Manufacturer	BioAssay Systems
Amount	100 Tests
Quantity options	100 Tests 100 x 100 Tests 20 x 100 Tests
Category	Other Assays & Kits Other Kits
Type	Assay-Kit
Specific against	other
Citations	Xue, H et al. (2020). Lactose-induced chronic diarrhea results from abnormal luminal microbial fermentation and disorder of ion transport in the colon. Frontiers in Physiology, 11, 877. Assay: Lactose in rat feces. Snyder, N. A., Palmer, M. V., Reinhardt, T. A., & Cunningham, K. W. (2019). Milk biosynthesis requires the Golgi cation exchanger TMEM165. Journal of Biological Chemistry, 294(9), 3181-3191. Assay: Lactose in mouse skimmed milk. Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44. Assay: Lactose in liposome. Kwon DH et al (2012).Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. J Nutr Biochem 23(7):791-9. Assay: Lactose in rat milk. To find more recent publications, please click here.
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Shipping Temperature	ICE
Storage Conditions	-20 °C
Description	For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Sensitive. Use as little as 20 µ L samples. Linear detection range in 96-well plate: 17 to 2000 µ M lactose for colorimetric assays and 6 to 100 µ M for fluorimetric assays. Convenient and high-throughput. Robust and homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: OD570nm, or FL530/585nm. Samples: Biological (milk), food and agriculture etc. Species: All. Procedure: Assay takes 30 min. Kit size: 100 tests. Detection limit: 6 µ M.
FAQs	I would like to know if I can use the optical density at 540nm to read my samples. The signal at 540nm is only about 2/3 of the signal at 570 nm, but you still can use it. However, you will lose sensitivity. For more detailed product information and questions, please feel free to send an inquiry to info@hoelzel.de
Overview	Application: For quantitative determination of lactose and evaluation of drug effects on lactose metabolism. Key Features: Use as little as 20 ?L samples. Linear detection range in 96-well plate: 17 to 2000 ?M lactose for colorimetric assays and 6 to 100 ?M for fluorimetric assays. Method: OD570nm, or FL530/585nm Samples: Biological (milk), food and agriculture etc Species: All Size: 100 tests Detection Limit: 6 ?M Shelf Life: 6 months More Details: Lactose (C₁₂H₂₂O₁₁), also called milk sugar, is a disaccharide that consists of ?-D-galactose and ?/?-D-glucose through a ?1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Shelf life (Months)	12

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

BAS-ELAC-100-datasheet.pdf

BAS-ELAC-100-safety-datasheet.pdf