EnzyChrom Lactose Assay Kit

Application

• For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

Key Features

• Use as little as 20 ?L samples. Linear detection range in 96-well plate: 17 to 2000 ?M lactose for colorimetric assays and 6 to 100 ?M for fluorimetric assays.

Method

• OD570nm, or FL530/585nm

Samples

• Biological (milk), food and agriculture etc

Species

• All

Size

• 100 tests

Detection Limit

• 6 ?M

Shelf Life

• 6 months

More Details

• Lactose (C₁₂H₂₂O₁₁), also called milk sugar, is a disaccharide that consists of ?-D-galactose and ?/?-D-glucose through a ?1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.