Comparison

EnzyChrom Lactose Assay Kit

Item no. BAS-ELAC-100
Manufacturer BioAssay Systems
Amount 100 Tests
Category
Type Assay-Kit
Specific against other
Citations Xue, H et al. (2020). Lactose-induced chronic diarrhea results from abnormal luminal microbial fermentation and disorder of ion transport in the colon. Frontiers in Physiology, 11, 877. Assay: Lactose in rat feces.

Snyder, N. A., Palmer, M. V., Reinhardt, T. A., & Cunningham, K. W. (2019). Milk biosynthesis requires the Golgi cation exchanger TMEM165. Journal of Biological Chemistry, 294(9), 3181-3191. Assay: Lactose in mouse skimmed milk.

Vabbilisetty, P and S Xue-Long (2014). Liposome surface functionalization based on different anchoring lipids via Staudinger ligation. Organic and Biomolecular Chemistry 12(8): 1237-44. Assay: Lactose in liposome.

Kwon DH et al (2012).Dietary protein restriction induces steatohepatitis and alters leptin/signal transducers and activators of transcription 3 signaling in lactating rats. J Nutr Biochem 23(7):791-9. Assay: Lactose in rat milk.


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ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Available
Description
Quantitative determination of lactose by colorimetric (570nm) or fluorimetric (530nm/590nm) methods. Procedure: 30 min. Kit size: 100 tests. Detection limit: 6 uM. Shelf life: 3 months. Shipping: on ice; storage: -20C.
Faqs

I would like to know if I can use the optical density at 540nm to read my samples.

The signal at 540nm is only about 2/3 of the signal at 570 nm, but you still can use it. However, you will lose sensitivity.


For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
Overview

Application

  • For quantitative determination of lactose and evaluation of drug effects on lactose metabolism.

Key Features

  • Use as little as 20 ?L samples. Linear detection range in 96-well plate: 17 to 2000 ?M lactose for colorimetric assays and 6 to 100 ?M for fluorimetric assays.

Method

  • OD570nm, or FL530/585nm

Samples

  • Biological (milk), food and agriculture etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • 6 ?M

Shelf Life

  • 6 months

More Details

  • Lactose (C12H22O11), also called milk sugar, is a disaccharide that consists of ?-D-galactose and ?/?-D-glucose through a ?1-4 glycosidic linkage. Lactose is the major sugar and makes up 2–8% of milk. Simple, direct and high-throughput assays for lactose determination find wide applications. BioAssay Systems assay uses specific enzyme-coupled reactions in which lactose is cleaved and the resulting galactose forms a colored product. The color intensity at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the lactose concentration in the sample.
Shipment
ICE
Storage
-20 C

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 Tests
Available: In stock
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