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Rat L2 Array, Membrane

Rat L2 Array, Membrane

Item no.	AAR-BLM-2-4
Manufacturer	Raybiotech
Amount	4 Sample Kit
Quantity options	2 Sample Kit 4 Sample Kit
Category	Arrays Protein Arrays
Type	Array
Specific against	Rat (Rattus norvegicus)
ECLASS 10.1	32161000
ECLASS 11.0	32161000
UNSPSC	41116126
Shipping condition	Cool pack
Available
Manufacturer - Applications	Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker Screening Identifying Key Factors Confirming a Biological Process Cross-species testing
Number of Targets Detected	500
Compatible Sample Types	Cell Culture Supernatants Cell Lysates Other Body Fluids Plasma Serum Tissue Lysates
Design Principle	Label-based
Method of Detection	Chemiluminescence
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	Membrane
Gene Symbols	Acaca \| Acp1 \| Acta2 \| Actc1 \| Actn1 \| Acvr1c \| Acvr2a \| Adam10 \| Adamts1 \| Adgrl3\|Â Adipoq \| Agrn \| Ak1 \| Alcam \| Andpro \| Angpt1 \| Anpep \| Anxa1 \| Anxa5 \| Apex1 \| Ar \| Arg1 \| Asah2 \| B2m \| Bad \| Bcam \| Bcl10 \| Bcl2l2 \| Bglap \| Bid \| Bmp2 \| Bst1 \| Btla \| C1qbp \| Cadm3 \| Cat \| Cav2 \| Ccdc22 \| Cckar \| Ccl11 \| Ccl27 \| Ccl3 \| Ccl5 \| Ccl7 \| Cd14 \| Cd200 \| Cd27 \| Cd276 \| Cd300lf \| Cd38 \| Cd48 \| Cd5 \| Cd63 \| Cd86 \| Cd8a \| Cd93 \| Cdc25b \| Cdc37 \| Cdh1 \| Cdh17 \| Cdh2 \| Cdh3 \| Cdh4 \| Cdkn1b \| Cdnf \| Ces1c \| Chrd \| Clu \| Cntf \| Cntn1 \| Cntn2 \| Cntn4 \| Cntnap5b \| Colec12 \| Cpa1 \| Cpa2 \| Cpb1 \| Creld2 \| Crkl \| Cryab \| Cst3 \| Ctgf \| Cthrc1 \| Ctsb \| Ctsc \| Ctse \| Ctsz \| Cubn \| Cxcl1 \| Cxcl10 \| Cxcl16 \| Cxcl9 \| Cycs \| Dcn \| Dhfr \| Dlk1 \| Dll1 \| Dnajb11 \| Dyrk3 \| Ecm1 \| Efna1 \| Efnb1 \| Egf \| Egfr \| Eif5a \| Eno1 \| Entpd2 \| Epha5 \| Ephb1 \| Ephb6 \| Epo \| Erbb2 \| Erbb3 \| Errfi1 \| Esr1 \| Ets1 \| F11r \| F2 \| Fabp2 \| Fabp3 \| Fabp5 \| Fetub \| Fgf1 \| Fgf12 \| Fgf2 \| Fgfr4 \| Fgr \| Fkbp1a \| Fkbp1b \| Fkbp4 \| Flt1 \| Fmod \| Frk \| Gabra4 \| Gad1 \| Gapdh \| Gas1 \| Gdf11 \| Gdnf \| Gfap \| Gfra1 \| Ggt1 \| Gh1 \| Git1 \| Glg1 \| Glo1 \| Glrx3 \| Got2 \| Gpc3 \| Gpt \| Gpx1 \| Gpx3 \| Grb2 \| Grin2a \| Grk1 \| Grk2 \| Grk5 \| Gzmb \| Haao \| Hagh \| Havcr1 \| Hgf \| Hif1a \| Hmox2 \| Hpx \| Hrg \| Hsd11b1 \| Hsp90ab1 \| Hspa1a \| Hspa9 \| Hspb1 \| Hspb6 \| Hspd1 \| Hsph1 \| Htra2 \| Ifng \| Igf1 \| Igfbp5 \| Il10 \| Il11ra1 \| Il12a \| Il13ra2 \| Il18 \| Il1b \| Il1r1 \| Il1r2 \| Il1rn \| Il2 \| Il21 \| Il22 \| Il23a \| Il2ra \| Il4 \| Il4r \| Il6 \| Il6st \| Il7 \| Il9 \| Ilk \| Ilkap \| Impdh1 \| Inppl1 \| Isl1 \| Jag1 \| Jam3 \| Kdr \| Keap1 \| Klkb1 \| Kng1 \| Lair1 \| Lamtor3 \| Lcn2 \| Lenep \| Lepr \| Lgals1 \| Lgals3 \| Lgals4 \| Lgmn \| Lif \| Lifr \| Lrp4 \| Lyn \| Lypd3 \| Maea \| Mag \| Maged1 \| Map2k2 \| Mapk12 \| Mapk9 \| Mbl2 \| Mcam \| Mif \| Mmp2 \| Mmp8 \| Mmp9 \| Mog \| Mospd1 \| Ncam1 \| Ncan \| Ncr3 \| Nedd8 \| Nes \| Nfasc \| Nfe2l2 \| Nfkbib \| Nlgn1 \| Nlgn2 \| Nope \| Nos1 \| Notch1 \| Notch2 \| Notch3 \| Nptn \| Nptx2 \| Nrgn \| Nrn1 \| Nrp1 \| Nrxn1 \| Ntn1 \| Ntrk1 \| Ntrk3 \| Nup85 \| Nxph1 \| Olfm1 \| Olr1 \| Pak1 \| Pak5 \| Pck1 \| Pdgfb \| Pdgfra \| Pecam1 \| Pfkm \| Pgc \| Pigr \| Plaur \| Pon3 \| Por \| Ppbp \| Ppia \| Ppib \| Ppm1a \| Ppp1r2 \| Ppp3r1 \| Prdx1 \| Prdx2 \| Prdx4 \| Prdx6 \| Prkaa2 \| Prkaca \| Prkca \| Prkce \| Prkci \| Prl \| Prl8a4 \| Procr \| Psap \| Psma1 \| Psma2 \| Ptk2 \| Ptpn1 \| Ptpn2 \| Ptpn6 \| Ptprf \| Rala \| Rbp4 \| Reg3a \| Reg3b \| Ren1 \| Rhd \| Robo1 \| Rock2 \| Rtn4 \| Scgn \| Sdc1 \| Sdc2 \| Sema4c \| Sema4d \| Sema5a \| Sema7a \| Serpina1 \| Serpine1 \| Serpinf1 \| Serpinf2 \| Sftpd \| Sh2b1 \| Slit3 \| Smad3 \| Smad7 \| Smarcd2 \| Smc1a \| Snca \| Sort1 \| Sost \| Sox10 \| Src \| Stat3 \| Stx1a \| Tafa5 \| Tagln \| Tek \| Tf \| Tgfbr3 \| Tnf \| Tnfrsf12a \| Tnfrsf1a \| Tnfrsf4 \| Tnfrsf9 \| Tollip \| Tp53 \| Tpt1 \| Trem1 \| Trhde \| Txn2 \| Uchl1 \| Uchl3 \| Unc5a \| Unc5b \| Vamp2 \| Vcl \| Vsig1 \| Wfdc2 \| Wnt5a \| Xiap \| Ywhae \| Ywhah \| Ywhaq
Kit Components	Ray BioÂ® L- Series Antibody Array Membranes Labeling Reagent Stop Solution Blocking Buffer Detection Buffer C Detection Buffer D Wash Buffer 1 Wash Buffer 2 H R P- Conjugated Streptavidin Concentrate Plastic Sheets Spin Columns Labeling Buffer Lysis Buffer Plastic Incubation Tray
Other Materials Required	Distilled or de-ionized water Small plastic or glass containers Orbital shaker or oscillating rocker Pipettors, pipette tips and other common lab consumables2?5 ml tube15 ml conical collection tube Tissue Paper, blotting paper or chromatography paper A chemiluminescent blot documentation system (such as U V P's Chemi Doc- ItÂ® or Epi Chem I I Benchtop Darkroom or G E's Image QuantÂ™ L A S 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline	Purify Samples with Spin Column Biotinylate Samples Purify biotinylated samples using spin column Block array membranes Incubate with Samples Incubate with H R P- Conjugated Streptavidin Incubate with Detection Buffers Image with chemiluminescent imaging system Perform densitometry and analysis
Product Features	High density arrays Unbiased detection through direct biotin labeling of samples Low sample consumption High detection sensitivity Same day result Affordable and simple to use
Storage/Stability	For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.