Antibody Details
Product Details
Reactivity Species
Mouse
Host Species
Rat
Immunogen
CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
⋅
> 95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
Flow Cytometry:For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed.
Other Applications Reported In Literature ?
IHC
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone 20D5 recognizes an epitope on the mouse NKG2A, NKG2C, and NKG2E isoforms of the CD94/NKG2 heterodimer. DBA/2J mice are CD94-deficient and do not express CD94/NKG2 receptors.
Antigen Distribution
NKG2A/C/E is expressed on NK cells, NKT cells, and activated CD8 T cells.
Background
NKG2A/C/E antibody, clone 20D5, recognizes the NKG2A, NKG2C, and NKG2E isoforms (also known as CD159a, CD159c, m CD159e, respectively) of the NKG2 receptor, belonging to the C-type lectin-like family. NKG2 receptors are expressed at the cell surface as a heterodimer with CD94 and recognize the non-classical class I MHC-1 molecules HLA-E in humans and Qa-1 in mice1-4. NKG2A/C/E are expressed on natural killer (NK) cells, NKT cells, and activated CD8 T cells5-6. Engagement of NKG2A/CD94 transduces an inhibitory signal, blocking NK and CD8 T cell cytotoxicity and promoting self-tolerance7. In contrast, NKG2C/CD94 and NKGE/CD94 are activating receptors8 and bind with lower affinity to HLA-E9. Cancer cells frequently overexpress HLA-E to protect against NK/CD8 T cell killing, and blocking NKG2 receptors in mice promotes anti-tumor immunity and may enhance the cytotoxic potential of other therapeutic antibodies10, 11. The NKG2 receptor antibody monalizumab is currently in phase III clinical trials (INTERLINK-1) in combination with cetuximab in patients with recurrent or metastatic squamous cell carcinoma of the head and neck.
Antigen Details
Protein
NKG2a
Molecular Weight
150 kDa
Function
Inhibitory activity via ITIMs
PubMed
NKG2a
Research Area
Cell Biology
.
Immunology
.
Signal Transduction
.
Stem Cell
References & Citations
1. Vance R.E., et al. (1998) J. Exp. Med. 188:1841–1848
2. Braud VM, et al. (1998) Nature. 391(6669):795-9
3. Vance RE, et al. (1999) J Exp Med. 190(12):1801-1812
4. Brooks AG, et al. (1997) J Exp Med. 185(4):795-800
5. Gunturi A, Berg RE, Forman J. (2004) Immunol Res. 30(1):29-34
6. Bertone S, et al. (1999) Eur J Immunol. 29(1):23-9
7. Le Dréan E, et al. (1998) Eur J Immunol. 28(1):264-76
8. Lanier LL, et al. (1998) Immunity. 8(6):693-701
9. Kaiser BK, et al. (2005) J Immunol. 174(5):2878-84
10. André P, Denis C, Soulas C, et al. (2018) Cell. 175(7):1731-1743.e13
11. van Montfoort N, Borst L, Korrer MJ, et al. (2018) Cell. 175(7):1744-1755.e15
Technical Protocols