Antibody Details
Product Details
Reactivity Species
Mouse
Host Species
Rat
Immunogen
Mouse Ly-6G transfected EL-4J cell line
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
> 95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
2-8°C
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
CyTOF®
Depletion
IHC (Frozen)
IHC (Paraffin)
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
Antigen Distribution
Ly6G is expressed by neutrophils.
Background
Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1, 4, 5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.
Antigen Details
Protein
Ly-6G
PubMed
Ly-6G
NCBI Gene Bank ID
546644
UniProt.org
Information on Uniprot.org
Immunology
.
Innate Immunity
References & Citations
1.) Fleming TJ, et al. (1993) J Immunol. 151(5):2399-408
2.) Tsetlin VI. et al. (2015) Trends Pharmacol Sci. 36(2):109-23
3.) Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70
4.) Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594
5.) Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328.
6.) Stroncek DF. et al. (2007) Curr Opin Hematol. 14(6):688-93
7.) Wang JX, et al. (2012) Blood. 120(7):1489-1498
8.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link
Technical Protocols