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Anti-Mouse Ly6G Antibody - Purified in vivo GOLD™ Functional Grade

Anti-Mouse Ly6G Antibody - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-L280-100mg
Manufacturer	Leinco Technologies
Amount	100 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IHC, IV
Clone	1A8
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1.) Fleming TJ, et al. (1993) J Immunol. 151(5):2399-408 2.) Tsetlin VI. et al. (2015) Trends Pharmacol Sci. 36(2):109-23 3.) Daley JM, et al. (2008) J Leukoc Biol. 83(1):64-70 4.) Lee PY, et al. (2013) J Leukoc Biol. 94(4):585-594 5.) Percopo CM, et al. (2017) J Leukoc Biol. 101(1):321-328. 6.) Stroncek DF. et al. (2007) Curr Opin Hematol. 14(6):688-93 7.) Wang JX, et al. (2012) Blood. 120(7):1489-1498 8.) Gubin et al. (2018) Cell. 175:1014–1030
NCBI	546644
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this AFS98 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|PhenoCycler®\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	Ly-6G
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	Ly6G antibody (clone 1A8) recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein1. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds2. Ly6G is expressed by murine neutrophils regardless of location and activation1, 4, 5. Eosinophils may also express low levels of Ly6G5. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia6. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism7.
Background	Ly6G antibody, clone 1A8, recognizes lymphocyte antigen 6 complex locus G6D (Ly6G; also called Gr-1), a 21-25 kDa glycosylphosphatidylinositol (GPI)-anchored protein¹. Ly6G belongs to the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) superfamily, characterized by a Ly6/uPAR (LU) domain-containing a three-fingered structural motif stabilized by disulfide bonds². Ly6G is expressed by murine neutrophils regardless of location and activation^{1, 4, 5}. Eosinophils may also express low levels of Ly6G⁵. There is no human ortholog for Ly6G; however, a structurally related L76/uPAR protein, CD177 (also known as HNA-2a, NB1, or PRV-1) is expressed in human neutrophils and is implicated in neutropenia⁶. Although the exact function and ligand of Ly6G remain unknown, Ly6G ligation may impair neutrophil migration to sites of inflammation via a β2-integrin-dependent mechanism⁷.
Other Applications Reported In Literature	CyTOF® Depletion IHC (Frozen) IHC (Paraffin) WB
PubMed	Ly-6G
Manufacturer - Research Area	Immunology, Innate Immunity
Manufacturer - Specificity	Clone 1A8 recognizes an epitope on mouse Ly6G. Clone 1A8 does not cross react with Ly6C.
RRID	AB_2737551
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Ly6G is expressed by neutrophils.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Mouse Ly-6G transfected EL-4J cell line
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-L280-100mg-safety-datasheet.pdf