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Anti-Human IL-4 - Purified In vivo GOLD™ Functional Grade

Anti-Human IL-4 - Purified In vivo GOLD™ Functional Grade

Item no.	LEIN-I-1163-5mg
Manufacturer	Leinco Technologies
Amount	5 mg
Quantity options	1 mg 100 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, IHC, IV
Clone	MP4-25D2
Specific against	other
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Available
Endotoxin	≤ 1.0 EU/mg
Product info	Antibody Details Product Details Reactivity Species Human Host Species Rat Immunogen CHO-expressed, recombinant human IL-4 Product Concentration ≥ 2.0 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC > 95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping 2-8°C RRID AB_2830503 Applications and Recommended Usage? Quality Tested by Leinco ELISA:Each lot of this antibody is quality control tested by ELISA. Additional Reported Applications For Relevant Conjugates ? N For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Clone MP4-25D2 recognizes human IL-4. Background IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients. Antigen Details Protein IL-4 PubMed IL-4 NCBI Gene Bank ID 3565 UniProt.org Information on Uniprot.org Research Area Cell Biology . Immunology . Neuroinflammation . Neuroscience References & Citations 1. Pike, JW. et al. (2001) PNAS 98: 2443 2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835 3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551 Technical Protocols

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1163-5mg-safety-datasheet.pdf