Antibody Details
Product Details
Reactivity Species
Human
Host Species
Rat
Immunogen
CHO-expressed, recombinant human IL-4
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
> 95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
2-8°C
RRID
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA:Each lot of this antibody is quality control tested by ELISA.
Additional Reported Applications For Relevant Conjugates ?
N
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Description
Specificity
Clone MP4-25D2 recognizes human IL-4.
Background
IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients.
Antigen Details
Protein
IL-4
PubMed
IL-4
NCBI Gene Bank ID
3565
UniProt.org
Information on Uniprot.org
Research Area
Cell Biology
.
Immunology
.
Neuroinflammation
.
Neuroscience
References & Citations
1. Pike, JW. et al. (2001) PNAS 98: 2443
2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835
3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551
Technical Protocols