Comparison

Anti-Mouse IFNγ (Clone XMG1.2) - Purified in vivo GOLD™ Functional Grade

Item no. LEIN-I-1119-50mg
Manufacturer Leinco Technologies
Amount 50 mg
Quantity options 1 mg 100 mg 25 mg 5 mg 50 mg
Category
Type Antibody Monoclonal
Applications WB, IHC, ELISA, IV
Clone XMG1.2
Specific against Mouse (Murine, Mus musculus)
Host Rat
Purity > 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations 1). Goeddel DV et al. (1982) Nature 298: 859
2.) Wilson CB et al. (2007) Adv. Immunol. 96: 41
3.) Hume DA et al. (2004) J Leukoc Biol. 75: 163
4.) Teijaro et al. (2020) bioRxiv 22: 24
5.) Hawman DW, et al. (2021) Microorganisms 9(2):279
NCBI 15978
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping condition Cool pack
Available
Manufacturer - Applications
Quality Tested by Leinco ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 μg/ml.
Manufacturer - Category
Purified in vivo Functional Grade>Gold Purified Bulk Antibodies|Primary Monoclonal Antibodies>Growth Factors and Cytokines|Purified in vivo Functional Grade
Manufacturer - Targets
IFN-γ
Country of Origin
USA
Shipping Temperature
Next Day 2-8°C
Storage Conditions
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3
Additional Reported Applications For Relevant Conjugates
IHC FF
WB
For specific conjugates of this clone, review literature for suggested application details.
Other Applications Reported In Literature
ICFC
ELISPOT Cap
ELISPOT Det
CyTOF®
N
PubMed
IFN-γ
Manufacturer - Research Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Manufacturer - Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID
AB_2737526
Concentration
≥ 5.0 mg/ml
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Endotoxin Level
≤ 1.0 EU/mg
Immunogen
Purified Recombinant Mouse IFN-γ (> 98%)
Additonal Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 mg
Available: In stock
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