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Anti-Mouse/Human IL-5 - Purified in vivo GOLD™ Functional Grade

Anti-Mouse/Human IL-5 - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-I-1060-50mg
Manufacturer	Leinco Technologies
Amount	50 mg
Quantity options	1 mg 100 mg 25 mg 50 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IHC, ELISA, FA, IV
Clone	TRFK5
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
NCBI	16191 3567
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC: The suggested concentration for this TRFK5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. NOTE: A fluorochrome-conjugated TRFK51 antibody would be useful for intracellular staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations. ELISA:This purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA assay. A suitable detection antibody would be a biotinylated clone TRFK4 antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	IL-5
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	This Low Endotoxin Functional Formulation, GOLD Antibody is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.
Background	IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.
Function	Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes
Other Applications Reported In Literature	NeutralizationThis Purified In vivo GOLD™ Functional Grade antibody (Endotoxin < 1.0 EU/mg) is recommended for neutralization of human IL-5 bioactivity. CyTOF® ELISA Cap FA IHC (Paraffin) WB Depletion
PubMed	IL-5
Manufacturer - Specificity	Clone TRFK5 recognizes mouse/human IL-5.
RRID	AB_2830393
Concentration	≥ 2.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Mast cells, T cells, eosinophils
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Partially-purified T cell clone supernatant
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-I-1060-50mg-safety-datasheet.pdf