Anti-Mouse/Human IL-5 - Purified In vivo GOLD™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Partially-purified T cell clone supernatant

Product Concentration

≥ 2.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

This Low Endotoxin Functional Formulation, GOLD Antibody is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2830393

Applications and Recommended Usage?
Quality Tested by Leinco

FC: The suggested concentration for this TRFK5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. NOTE: A fluorochrome-conjugated TRFK51 antibody would be useful for intracellular staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations.
ELISA:This purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA assay. A suitable detection antibody would be a biotinylated clone TRFK4 antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody.

Other Applications Reported In Literature ?

NeutralizationThis Purified In vivo GOLD™ Functional Grade antibody (Endotoxin < 1.0 EU/mg) is recommended for neutralization of human IL-5 bioactivity. CyTOF®
ELISA Cap
FA
IHC (Paraffin)
WB
Depletion

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Clone TRFK5 recognizes mouse/human IL-5.

Antigen Distribution

Mast cells, T cells, eosinophils

Background

IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma.

Antigen Details

Protein

IL-5

Function

Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes

PubMed

IL-5

NCBI Gene Bank ID

16191 3567

UniProt.org

Information on Uniprot.org

Technical Protocols