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Anti-Human HLA-A, B, C (MHC Class I) - Purified in vivo GOLD™ Functional Grade

Anti-Human HLA-A, B, C (MHC Class I) - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-H263-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IP, IHC, other, IV
Clone	W6/32
Specific against	Human (Homo sapiens), Monkey (Cynomolgus, Simian), Cat (Feline, Felis catus), Cattle (Bovine)
Host	Mouse
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25 2. Wieczorek M, et al. (2017) Front Immunol. 8:292 3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9 4. Matsumura M, et al. (1992) Science. 257:927–34.10.1126/science.1323878 5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162 6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743 7. Cruz FM, et al (2017) Annu Rev Immunol. 35:149-176 8. Artyomov MN, et al (2010) Proc Natl Acad Sci USA. 107(39):16916-16921 9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856 10. Raulet DH. (1994) Adv Immunol. 55:381-421 11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58
NCBI	3105
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this HLA-A, B, C Clone W6/32 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 10⁶ cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for for use in western blotting is 1-10 μg/ml.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Human MHC\|PhenoCycler®
Manufacturer - Targets	HLA-A, B, C
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Product Description	HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1, 2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10, 11.
Background	HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)^{1, 2}. The α-chain consists of three domains (α1, α2, and α3)³. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens^4-6. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation⁷. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling⁸. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance⁹. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity^{10, 11}.
Function	Antigen presentation
Other Applications Reported In Literature	B CODEX® IHC FF IP
PubMed	HLA-A, B, C
Ligand/Receptor	CD3/TCR, CD8
Manufacturer - Research Area	Immunology, Innate Immunity
Manufacturer - Specificity	Clone W6/32 recognizes the human MHC class I molecules HLA-A, -B, and -C.
RRID	AB_2737521
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	HLA-A, -B, and -C are ubiquitously expressed on nucleated cells.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Human tonsil cell membrane
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-H263-1.0mg-safety-datasheet.pdf