Anti-Mouse Ly-6G/Ly-6C (Gr-1) - Purified In vivo GOLD™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Raised against granulocytes of mouse origin

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737495

Applications and Recommended Usage?
Quality Tested by Leinco

FC The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 μg per 10⁶ cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

CyTOF®
Depletion Clone RB6-8C5 is suitable for In vivo depletion. However, it has been reported that clone RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs).
IHC (Frozen)
IHC (Paraffin)
IP
WB

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1, 2

Background

Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.

Antigen Details

Protein

Ly-6G/Ly-6C

PubMed

Ly-6G/Ly-6C

NCBI Gene Bank ID

17067 546644

UniProt.org

Information on Uniprot.org

Immunology

.

Innate Immunity

References & Citations

1.) Yokoyama, Hitoshi et al. J Am Soc Nephrol. 2003 Oct; 14(10):2503-15. PubMed
2.) Fleming, T. J. et al. (1993) J. of Immunol. 151(5):2399
3.) Hestdal, K. et al. (1991) J. of Immunol. 147(1):22
4.) Brummer, E. et al. (1984) J. Leuko. Bio. 36:505

Technical Protocols