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Anti-Mouse Ly-6G/Ly-6C (Gr-1) (Clone RB6-8C5) - Purified in vivo GOLD™ Functional Grade

Anti-Mouse Ly-6G/Ly-6C (Gr-1) (Clone RB6-8C5) - Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-G100-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, IP, IHC, IV
Clone	RB6-8C5
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1.) Yokoyama, Hitoshi et al. J Am Soc Nephrol. 2003 Oct;14(10):2503-15. 2.) Fleming, T. J. et al. (1993) J. of Immunol. 151(5):2399 3.) Hestdal, K. et al. (1991) J. of Immunol. 147(1):22 4.) Brummer, E. et al. (1984) J. Leuko. Bio. 36:505
NCBI	17067 546644
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this RB6-8C5 antibody for staining cells in flow cytometry is ≤ 0.06 μg per 10⁶ cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	Ly-6G/Ly-6C
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.
Background	Gr-1 is a 21-25 kD protein. This myeloid differentiation antigen is a glycosylphosphatidylinositol-linked protein expressed on granulocytes and macrophages. Clone RB6-8C5 antibody has been shown to inhibit the binding of the clone 1A8 antibody. Clone 1A8 monoclonal antibody reacts specifically with mouse Ly6G with no reported cross-reactivity with Ly6C.
Other Applications Reported In Literature	CyTOF® Depletion Clone RB6-8C5 is suitable for In vivo depletion. However, it has been reported that clone RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs). IHC (Frozen) IHC (Paraffin) IP WB
PubMed	Ly-6G/Ly-6C
Manufacturer - Research Area	Immunology, Innate Immunity
Manufacturer - Specificity	Rat Anti-Mouse GR-1, as known as anti-mouse Ly-6G/Ly-6C (Clone RB6-8C5) has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2737495
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	The Gr-1 antigen is present at various levels correlated with granulocyte differentiation and maturation. The Gr-1 antigen is expressed on other myeloid populations, but not on lymphoid or erythroid cells.1, 2
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Raised against granulocytes of mouse origin
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-G100-1.0mg-safety-datasheet.pdf