Comparison

Anti-Mouse CD54 (Clone YN1/1.7.4) - Purified in vivo PLATINUM™ Functional Grade

Item no. LEIN-C6391-5.0mg
Manufacturer Leinco Technologies
Amount 5 mg
Quantity options 1 mg 100 mg 25 mg 5 mg 50 mg
Category
Type Antibody Monoclonal
Applications WB, FC, IP, IHC, FA, IV
Clone YN1/1.7.4
Specific against Mouse (Murine, Mus musculus)
Host Rat
Purity > 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC
Citations 1. Li, S. et al. (2009) Biochem. Biophys. Res. Commun. 381: 459 2. Wolf, S. et al. (2009) Pharmacol. Rep. 61: 22 3. Ozcan, U. et al. (2009) Arch Gynecol. Obstet.
NCBI 15894
ECLASS 10.1 42030590
ECLASS 11.0 42030590
UNSPSC 12352203
Shipping condition Cool pack
Available
Manufacturer - Applications
Quality Tested by Leinco FC The suggested concentration for this YN1/1.7.4 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this YN1/1.7.4 antibody for use in western blotting is 1-10 μg/ml.
Manufacturer - Category
PhenoCycler®|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies|Purified in vivo Functional Grade|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets
CD54
Country of Origin
USA
Shipping Temperature
Next Day 2-8°C
Storage Conditions
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.
Background
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Function
Immune reaction, inflammation, adhesion
Additional Reported Applications For Relevant Conjugates
B
IHC (Frozen)
For specific conjugates of this clone, review literature for suggested application details.
Other Applications Reported In Literature
CODEX®
CyTOF®
IP
PubMed
CD54
Ligand/Receptor
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen
Manufacturer - Research Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cel
Manufacturer - Specificity
Clone YN1/1.7.4 recognizes an epitope on mouse CD54.
RRID
AB_2829795
Concentration
≥ 5.0 mg/ml
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution
CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes.
Endotoxin Level
≤ 0.5 EU/mg
Immunogen
Mouse NS-1 cells
Additonal Information
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 5 mg
Available: In stock
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