Item no. |
LEIN-C6391-1.0mg |
Manufacturer |
Leinco Technologies
|
Amount |
1 mg |
Quantity options |
1 mg
100 mg
25 mg
5 mg
50 mg
|
Category |
|
Type |
Antibody Monoclonal |
Applications |
WB, FC, IP, IHC, FA, IV |
Clone |
YN1/1.7.4 |
Specific against |
Mouse (Murine, Mus musculus) |
Host |
Rat |
Purity |
> 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC |
Citations |
1. Li, S. et al. (2009) Biochem. Biophys. Res. Commun. 381: 459 2. Wolf, S. et al. (2009) Pharmacol. Rep. 61: 22 3. Ozcan, U. et al. (2009) Arch Gynecol. Obstet. |
NCBI |
15894 |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Shipping condition |
Cool pack |
Available |
|
Manufacturer - Applications |
Quality Tested by Leinco FC The suggested concentration for this YN1/1.7.4 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this YN1/1.7.4 antibody for use in western blotting is 1-10 μg/ml. |
Manufacturer - Category |
PhenoCycler®|Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies|Purified in vivo Functional Grade|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers |
Manufacturer - Targets |
CD54 |
Country of Origin |
USA |
Shipping Temperature |
Next Day 2-8°C |
Storage Conditions |
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. |
Product Description |
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies. |
Background |
ICAM-1 is a 55 kDa glycoprotein that is part of the Ig superfamily. It is heavily glycosylated to form 75 kDa to 115 kDa. ICAM-1 is known to be an adhesion and viral entry molecule, and its long suspected involevement in signal transduction is being elucidated. The signal-transducing functions of ICAM-1 appear to be mainly associated with proinflammatory pathways. Furthermore, ICAM-1 signaling appears to act as a beacon for inflammatory immune cells such as macrophages and granulocytes bringing about inflammation via lymphocyte trafficking. ICAM-1 is essential for the transmigration of leukocytes out of blood vessels and into tissues, and is a marker of endothelial dysfunction leading to damaging vascular disorders in umbilical and placental vascular tissue of gestational pregnancies. ICAM-1 is the receptor for rhinoviruses (the cause of most common colds) and malaria, and plays an inflammatory role in ocular allergies. |
Pathogen Testing |
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. |
Function |
Immune reaction, inflammation, adhesion |
Additional Reported Applications For Relevant Conjugates |
B IHC (Frozen) For specific conjugates of this clone, review literature for suggested application details. |
Other Applications Reported In Literature |
CODEX® CyTOF® IP |
PubMed |
CD54 |
Ligand/Receptor |
CD11a/CD18 (LFA-1) or CD11b/CD18 (Mac-1) and CD11c/CD18, CD43, hyaluronan, fibrinogen |
Manufacturer - Research Area |
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cel |
Manufacturer - Specificity |
Clone YN1/1.7.4 recognizes an epitope on mouse CD54. |
RRID |
AB_2829795 |
Concentration |
≥ 5.0 mg/ml |
Formulation |
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. |
Product Preparation |
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. |
Antigen Distribution |
CD54 is present on endothelial cells, lymphocytes, epithelial cells, dendritic cells and keratinocytes. |
Endotoxin Level |
≤ 0.5 EU/mg |
Immunogen |
Mouse NS-1 cells |
Additonal Information |
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.