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Anti-Mouse CD62L (Clone MEL-14)- Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD62L (Clone MEL-14)- Purified in vivo PLATINUM™ Functional Grade

Item no.	LEIN-C6118-25mg
Manufacturer	Leinco Technologies
Amount	25 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	WB, FC, IP, IHC, other, IV
Clone	MEL-14
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 98% monomer by analytical SEC
NCBI	20343
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this MEL-14 antibody for use in western blotting is 1-10 μg/ml.
Manufacturer - Category	Purified in vivo Functional Grade>Platinum Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD62L
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.
Background	CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody.
Pathogen Testing	To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Function	Lymphocyte homing to HEV, rolling on activated endothelium
Additional Reported Applications For Relevant Conjugates	IHC (Paraffin) For specific conjugates of this clone, review literature for suggested application details.
Other Applications Reported In Literature	CyTOF® IHC FF IP B
PubMed	CD62L
Ligand/Receptor	CD34, GlyCAM-1, MAdCAM-1
Manufacturer - Research Area	Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity
Manufacturer - Specificity	Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2829778
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Subsets of B and T cells, monocytes, granulocytes, subset of NK cells
Endotoxin Level	≤ 0.5 EU/mg
Immunogen	C3H/eb mouse B lymphoma 38C-13
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C6118-25mg-safety-datasheet.pdf