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Anti-Mouse CD3 - Purified In vivo GOLD™ Functional Grade

Anti-Mouse CD3 - Purified In vivo GOLD™ Functional Grade

Item no.	LEIN-C2443-5.0mg
Manufacturer	Leinco Technologies
Amount	5 mg
Quantity options	0.5 mg 1 mg 5 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IHC, IV
Clone	17A2
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1. Gallimore, A. et al. (2005) J Immunol. 175(11):7098-102. 2. Mysliwietz, J. et al. (1992) Blood 80: 2661-2667
NCBI	12502
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco FC The suggested concentration for this 17A2 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Country of Origin	USA
Shipping Temperature	2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Background	CD3ε is a 20kDa subunit of the TCR complex and is a transmembrane T-cell surface glycoprotein that belongs to the Ig superfamily. It is one of five polypeptide chains that form the TCR complex by associating with the CD3δ, γ and ζ chains, in addition to the TCR α/β or γ/δ chains. CD3 is involved in TCR signaling, enumeration of immunocompetent T-lymphocytes in peripheral blood, and signal transduction during antigen recognition.
Function	Antigen recognition, TCR signal transduction, T cell activation
Other Applications Reported In Literature	CODEX® IHC (Frozen) The suggested concentration for this 17A2 antibody in IHC staining on frozen tissue is 5.0 - 10 μg per ml. Titration of the reagent is recommended for optimal performance for each application.
PubMed	CD3
Ligand/Receptor	Peptide antigen/MHC-complex
Manufacturer - Specificity	Clone 17A2 recognizes an epitope on mouse CD3.
RRID	AB_2737469
Concentration	≥1.0 mg/ml
Formulation	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	CD3 is expressed on human peripheral blood lymphocytes, splenic lymphocytes, and the majority of T-CLL and T-ALL cells.
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	γ/δ TCR-positive T-T hybridoma D1
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C2443-5.0mg-safety-datasheet.pdf