Anti-Mouse CD275 - Purified In vivo GOLD™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Transfected Cell Line for Mouse B7H2

Product Concentration

≥ 2.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2829462

Applications and Recommended Usage?
Quality Tested by Leinco

Flow CytometryFor flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µL volume or 100 µL of whole blood.

Other Applications Reported In Literature ?

Additional reported applications (for the relevant formats) include:
Blocking ligand binding

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse B7-H2 (CD275) (Clone HK5.3) recognizes an epitope on Mouse CD275. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

splenic B-cells, T-cells, dendritic cells and macrophages

Background

B7-H2, also known as ICOSL and B7RP-1, is a member of the B7 family of co-stimulatory molecules related to B7-1 and B7-2. It is a transmembrane glycoprotein with extracellular IgV and IgC domains. B7-H2 is expressed on B cells and macrophages. It binds to ICOS on activated T cells and thus delivers a positive costimulatory signal for optimal T cell function (1). ICOS and B7RP-I do not interact with proteins in the CD28-B7 pathway (2). The structural features of B7-H2 are crucial for its costimulatory function. B7-H2 constructs lacking either the IgC or IgV domain demonstrates that receptor binding is mediated solely by the IgV domain but requires the IgC domain for maintaining the structural integrity of the protein (3).

Antigen Details

Protein

B7-H2

Ligand/Receptor

ICOS

Function

Binds to ICOS. Co-stimulates T cell responses including proliferation and cytokine secretion. Stimulates B cell proliferation and differentiation to plasma cells.

PubMed

B7-H2

NCBI Gene Bank ID

50723

UniProt.org

Information on Uniprot.org

References & Citations

1. Wiendl, H. et al. (2003) Brain 126:1026
2. Yoshinaga, SK. et al. (1999) Nature 402:827
3. Chattopadhyay, KJ. et al. (2006) Immunol. 177:3920

Technical Protocols