Anti-Mouse CD4 (Clone GK1.5) - Purified In vivo GOLD™ Functional Grade

Antibody Details

Product Details

Reactivity Species

Mouse

Host Species

Rat

Immunogen

Mouse CTL clone V4

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

≤ 1.0 EU/mg as determined by the LAL method

Purity

≥95% monomer by analytical SEC

> 95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

2-8°C

RRID

AB_2737452

Applications and Recommended Usage?
Quality Tested by Leinco

CyTOF®
FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.

Other Applications Reported In Literature ?

B
Costim
Depletion
IHC
IP

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution

Majority of thymocytes, T cell subset

Background

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.⁶

Antigen Details

Protein

CD4

Ligand/Receptor

MHC class II molecule

Function

T cell activation

PubMed

CD4

NCBI Gene Bank ID

12504

UniProt.org

Information on Uniprot.org

References & Citations

1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed
2.) Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
3.) Nicolas, JF. et al. (2002) J Immunol.168(6):3079-87. Article Link
4.) Shin, H. et al. (2018) J Virol. 92(7): e00038-18. PubMed
5.) Chiang, BL. et al. (2001) Immunology. 2001 103(3): 301–309. PubMed
6.) Hendrickson, WA. et al. (1994) Structure 2: 59
7.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5 Journal Link
8.) Raju et al. (2019) Cell Reports. 29:2556–2564 Journal Link
9.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link
10.) Sharma S. et al. (2020) Human Vaccines & Immunotherapeutics 16(9):2196-2203 Journal Link
11.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link

Technical Protocols