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Anti-Mouse CD4 Antibody (Clone GK1.5) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CD4 Antibody (Clone GK1.5) – Purified in vivo GOLD™ Functional Grade

Item no.	LEIN-C1333-1.0mg
Manufacturer	Leinco Technologies
Amount	1 mg
Quantity options	1 mg 100 mg 25 mg 5 mg 50 mg
Category	In Vivo Antibodies In Vivo Antibodies
Type	Antibody Monoclonal
Applications	FC, IP, IHC, FA, other, IV
Clone	GK1.5
Specific against	Mouse (Murine, Mus musculus)
Host	Rat
Purity	> 95% by SDS-PAGE and ≥ 95% monomer by analytical SEC
Citations	1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. 2.) Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. 3.) Nicolas, JF. et al. (2002) J Immunol.168(6):3079-87. 4.) Shin, H. et al. (2018) J Virol. 92(7): e00038-18. 5.) Chiang, BL. et al. (2001) Immunology. 2001 103(3): 301–309. 6.) Hendrickson, WA. et al. (1994) Structure 2: 59 7.) Skyberg, J. A. et al. (2020) Infection and Immunity. 88: 5 8.) Raju et al. (2019) Cell Reports. 29:2556–2564 9.) Gubin et al. (2018) Cell. 175:1014–1030 10.) Sharma S. et al. (2020) Human Vaccines & Immunotherapeutics 16(9):2196-2203 11.) Hawman DW, et al. (2021) Microorganisms 9(2):279
NCBI	12504
ECLASS 10.1	42030590
ECLASS 11.0	42030590
UNSPSC	12352203
Shipping condition	Cool pack
Available
Manufacturer - Applications	Quality Tested by Leinco CyTOF® FC The suggested concentration for this GK1.5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 10⁶ cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Manufacturer - Category	Purified in vivo Functional Grade>Gold Purified Bulk Antibodies\|Purified in vivo Functional Grade\|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
Manufacturer - Targets	CD4
Country of Origin	USA
Shipping Temperature	Next Day 2-8°C
Storage Conditions	Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Product Description	CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.6
Background	CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.⁶
Function	T cell activation
Other Applications Reported In Literature	B Costim Depletion IHC IP
PubMed	CD4
Ligand/Receptor	MHC class II molecule
Manufacturer - Specificity	Rat Anti-Mouse CD4 (Clone GK1.5) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
RRID	AB_2737452
Concentration	≥ 5.0 mg/ml
Formulation	This monoclonal CD4 antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Antigen Distribution	Majority of thymocytes, T cell subset
Endotoxin Level	≤ 1.0 EU/mg
Immunogen	Mouse CTL clone V4
Additonal Information	Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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LEIN-C1333-1.0mg-safety-datasheet.pdf