Comparison

Rabbit anti-HSF1 (pS307) Antibody

Item no. YLD4135-100ul
Manufacturer YL Biotech
Amount 100ul
Category
Type Antibody Polyclonal
Applications WB, IF, IC
Specific against Human
Host Rabbit
ECLASS 10.1 32160702
ECLASS 11.0 32160702
UNSPSC 12352203
Alias HSTF1; Heat shock factor protein 1; HSF 1; Heat shock transcription factor 1; HSTF 1
Available
Background
Rabbit polyclonal antibody to HSF1 (pS307)
Concentration
1mg/mL
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human HSF1. The exact sequence is proprietary.
WB description
Western blot analysis of HSF1 (pS307) expression in HepG2 (A), K562 (B) whole cell lysates.
IF/ICC description
Immunofluorescent analysis of HSF1 (pS307) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Purification_Method
The antibody was purified by immunogen affinity chromatography.
Clonality
Polyclonal
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution
WB (1/500 - 1/1000), IF/IC (1/100 - 1/500)
Storage
Store at -20℃. Avoid repeated freeze / thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100ul
Available: In stock
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