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Rabbit anti-cTnI Antibody

Rabbit anti-cTnI Antibody

Item no.	YLD2240-50ul
Manufacturer	YL Biotech
Amount	50ul
Category	Antibodies Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IF, IHC, IC
Specific against	Mouse, Rat, Rabbit, Pig
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	TNNI3; TNNC1; Troponin I, cardiac muscle; Cardiac troponin I
Available
Background	Rabbit polyclonal antibody to cTnI
Concentration	1mg/mL
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human cTnI. The exact sequence is proprietary.
WB description	Western blot analysis of over-expressed FLAG-tagged protein in 293T cell lysate.
IF/ICC description	Immunofluorescent analysis of FLAG-tag staining in 293T cells transfected with a Flag-tag protein. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
IP description	Immunoprecipitation of FLAG-tagged protein from HEK293T cells transfected with vector overexpressing Flag tag, using anti-FLAG-tag Antibody.
Clonality	Monoclonal
Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution	WB (1/2000 - 1/5000), IF/IC (1/200 - 1/500), IP (1/100 - 1/200)
Storage	Store at -20℃. Avoid repeated freeze / thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 50ul

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Delivery expected until 9/13/2024

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