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Rabbit anti-AS160 (pT642) Antibody

Rabbit anti-AS160 (pT642) Antibody

Item no.	YLD0602-100ul
Manufacturer	YL Biotech
Amount	100ul
Category	Antibodies Primary Antibodies
Type	Antibody Polyclonal
Applications	WB, IF, IHC, IC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	AS160; KIAA0603; TBC1 domain family member 4; Akt substrate of 160 kDa; AS160
Available
Background	Rabbit polyclonal antibody to AS160 (pT642)
Concentration	1mg/mL
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the center region of human AS160. The exact sequence is proprietary.
WB description	Western blot analysis of AS160 (pT642) expression in LO2 insulin-treated (A), Raw264.7 insulin-treated (B), PC12 insulin-treated (C), NIH3T3 UV-treated (D) whole cell lysates.
IHC description	Immunohistochemical analysis of AS160 (pT642) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description	Immunofluorescent analysis of AS160 (pT642) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Purification_Method	The antibody was purified by immunogen affinity chromatography.
Clonality	Polyclonal
Buffer	Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution	WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/100 - 1/500)
Storage	Store at -20℃. Avoid repeated freeze / thaw cycles.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100ul

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Delivery expected until 9/13/2024

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