Item no. |
YLD0183-100ul |
Manufacturer |
YL Biotech
|
Amount |
100ul |
Category |
|
Type |
Antibody Polyclonal |
Applications |
WB, IF, IHC, IC |
Specific against |
Human (Homo sapiens), Mouse (Murine, Mus musculus), Rat (Rattus norvegicus) |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Adenylate kinase isoenzyme 1; AK 1; ATP-AMP transphosphorylase 1; ATP:AMP phosphotransferase; Adenylate monophosphate kinase; Myokinase |
Available |
|
Background |
Rabbit polyclonal antibody to Adenylate Kinase 1 |
Concentration |
1mg/mL |
Immunogen |
Recombinant full length protein of human Adenylate Kinase 1 |
WB description |
Western blot analysis of Adenylate Kinase 1 expression in mouse heart (A) whole cell lysates. |
IHC description |
Immunohistochemical analysis of Adenylate Kinase 1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. |
IF/ICC description |
Immunofluorescent analysis of Adenylate Kinase 1 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. |
Purification_Method |
The antibody was purified by immunogen affinity chromatography. |
Clonality |
Polyclonal |
Buffer |
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
Dilution |
WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) |
Storage |
Store at -20℃. Avoid repeated freeze / thaw cycles. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.