Anti-Histone Deacetylase 1 antibody (C-Term) (STJ70893)

Pep-ELISA/WB/IF/ChIP

Store at-20°C on receipt and minimise freeze-thaw cycles.

0.5 mg/mL

Polyclonal

Peptide ELISA: antibody detection limit dilution 1:4000.
WB: Appox 55kDa band observed in Human Duodenum and Lymph Node lysates and approx 70kDa band observed in lysates of cell lines HeLa and NIH3T3 (calculated MW of 55.1kDa according to Human NP_004955.2 and Mouse NP_032254.1). The observed 70kDa band represents the sumoylated protein according to David et al, J Biol Chem. 2002 Jun 28; 277 (26) :23658-63; PMID: 11960997. Recommended concentration: 0.1-2µg/ml.

IF: Strong expression of the protein seen in the nucleus of NIH3T3 cells. Recommended concentration: 10µg/ml

Chromatin Immunoprecipitation: A 3.5-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB07186 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.

C-Term