MIP-4 Antibody

Chemokines & Cytokines, Antibody Pairs, Functional Assays

Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hMIPu4 (100.0 ng/mL), a concentration of 8.0 - 10.0 μ g/mL of this antibody is required.

ELISA:

To detect hMIP-4 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-4.

Sandwich:

To detect hMIP-4 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-4 (XP-5242Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4.

Western Blot:
To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

MIP-4 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

None

P55774

6362

CCL18

Homo sapiens

To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 buffer is recommended. Optimal concentrations and conditions may vary.