Item no. |
PRS-XP-5242-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
CKb7, PARC, AMAC1, DCCK1, MIP-4, AMAC-1, DC-CK1, SCYA18, MIP4, C-C motif chemokine 18, Alternative macrophage activation-associated CC chemokine 1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Chemokines & Cytokines, Antibody Pairs, Functional Assays |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hMIP-4 (human Macrophage Inflammatory Protein-4). |
Applications |
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMIPu4 (100.0 ng/mL), a concentration of 8.0 - 10.0 μ g/mL of this antibody is required.
ELISA:
To detect hMIP-4 by direct ELISA (using 100 μ L/well antibody solution) a concentration of at least 0.5 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hMIP-4.
Sandwich:
To detect hMIP-4 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.5 - 2.0 μ g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human MIP-4 (XP-5242Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMIP-4.
Western Blot: To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Purification |
Anti-hMIP-4 specific antibody was purified by affinity chromatography employing immobilized hMIP-4 matrix. |
Clonality |
Polyclonal |
Conjugate |
Unconjugated |
Storage Conditions |
MIP-4 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CCL18 |
Accession # |
P55774 |
Protein Gi # |
2493666 |
Ncbi Gene Id # |
6362 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
CCL18 |
Ncbi Official Full Name |
chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P55774 |
1st Image Caption |
To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
2nd Image Caption |
To detect hMIP-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIP-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
3rd Image Caption |
This antibody stained formalin-fixed, paraffin-embedded sections of human normal placenta. The recommended concentration is 5.0 ug/ml with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 buffer is recommended. Optimal concentrations and conditions may vary. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.