IL-10 Antibody (biotin)

Immunology, Chemokines & Cytokines, Antibody Pairs

ELISA:

Sandwich:
To detect Rat IL-10 by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Rat IL-10 (XP-5163) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-10.

Western Blot:
To detect rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Polyclonal

IL-10 antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles.

None

P29456

25325

Il10

Rattus norvegicustus norvegicus

Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL.

To detect Rat IL-10 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.