Item no. |
PRS-XP-5136Bt-0.05mg |
Manufacturer |
ProSci
|
Amount |
0.05 mg |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
AFGF, ECGF, FGFA, ECGFA, ECGFB, FGF-1, HBGF1, HBGF-1, GLIO703, ECGF-beta, FGF-alpha, Fibroblast growth factor 1, Acidic fibroblast growth factor |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Growth Factor, Antibody Pairs |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hFGF-acidic (human FGF-acidic). |
Applications |
ELISA:
Sandwich: To detect hFGF-acidic by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human FGF-acidic (XP-5136) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hFGF-acidic.
Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Purification |
Anti-hFGF-acidic specific antibody was purified by affinity chromatography and then biotinylated. |
Clonality |
Polyclonal |
Conjugate |
Biotin |
Storage Conditions |
FGF-acidic antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
FGF1 |
Accession # |
P05230 |
Protein Gi # |
122737 |
Ncbi Gene Id # |
2246 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
FGF1 |
Ncbi Official Full Name |
fibroblast growth factor 1 (acidic) |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P05230 |
1st Image Caption |
To detect hFGF-acidic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human FGF-acidic (XP-5136) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hFGF-acidic. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.