Item no. |
PRS-XP-5115Bt-0.05mg |
Manufacturer |
ProSci
|
Amount |
0.05 mg |
Category |
|
Type |
Antibody Primary |
Format |
Lyophilized |
Applications |
WB, ELISA |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
HCNTFCiliary neurotrophic factorCNTF |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Chemokines & Cytokines, Antibody Pairs |
Immunogen |
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCNTF (human CNTF). |
Applications |
ELISA:
Sandwich: To detect hCNTF by sandwich ELISA (using 100 μ L/well antibody solution) a concentration of 0.25 - 1.0 μ g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human CNTF (XP-5115) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCNTF.
Western Blot: To detect hCNTF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCNTF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Purification |
Anti-hCNTF specific antibody was purified by affinity chromatography and then biotinylated. |
Clonality |
Polyclonal |
Conjugate |
Biotin |
Storage Conditions |
CNTF antibody is stable for at least 2 years from date of receipt at -20˚ C. The reconstituted antibody is stable for at least two weeks at 2-8˚ C. Frozen aliquots are stable for at least 6 months when stored at -20˚ C. Avoid repeated freeze-thaw cycles. |
Disclaimer |
This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
CNTF |
Accession # |
P26441 |
Protein Gi # |
116585 |
Ncbi Gene Id # |
1270 |
User Note |
Centrifuge vial prior to opening. |
Ncbi Official Symbol |
CNTF |
Ncbi Official Full Name |
ciliary neurotrophic factor |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P26441 |
Background |
Ciliary neurotrophic factor (CNTF) is expressed in glial cells within the central and peripheral nervous systems. CNTF stimulates gene expression, cell survival or differentiation in a variety of neuronal cell types such as sensory, sympathetic, ciliary and motor neurons. CNTF itself lacks a classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release from adult glial cells by some mechanism induced by injury. |
1st Image Caption |
To detect hCNTF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human CNTF (XP-5115) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hCNTF. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.