B7-H3 Detection Set (Risk Free)

PBS containing 0.02% sodium azide.

Antibodies can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

B7-H3 Antibody: CD276, also known as B7-H3, was initially identified as a member of the B7 family of proteins through its homology with previously identified B7 molecules (1). CD276 mRNA is widely expressed, but its protein expression is usually rather low (2). CD276 has been shown to play a role in both the costimulation as well as the coinhibition of T cell response (3). In a similar fashion, CD276 plays a critical role in the control of antitumor immune responses in some cases, while in others appears to mediate antitumor immunity (4). It thus joins other immune checkpoint proteins as a possible therapeutic target for at least a subset of cancers. ProSci's Risk-FreeTM antibodies are mouse monoclonal antibodies made to improve in vivo studies. Unlike antibodies developed using proteins made in yeast or bacteria, Risk-FreeTM antibodies are developed with antigens expressed in mammalian cell lines, giving the most native post-translational modifications to the protein. Validated for flow cytometry and ELISA Rigorously tested for the following applications: Immunoblot Immunohistochemistry Immunocytochemistry Immunofluorescence Multiple antibodies per target allowing the user to choose the best antibody for their application Available individually or as a set Risk-FreeTM means they are guaranteed to work for their approved applications

B7-H3 antibodies were raised against the extracellular domain of human B7-H3

Monoclonal

Chapoval AI, Ni J, Lau JS, et al. B7-H3: a costimulatory molecule for T cell activation and IFN-gamma production. Nat Immunol 2001; 2:269-74.

Yi KH and Chen L. Fine tuning the immune response through B7-H3 and B7-H4. Immunol Rev 2009; 229:145-51.

RF16091, RF16092, RF16093, RF16094, RF16095

Western blot analysis of B7-H3 in HEK293 cells using RF16091, RF16092, and RF16093 antibodies at (A) 0.25 mg/ml , (B) 0.5 mg/ml, and (C) 1 μ g/ml.

Immunofluorescence of B7-H3 in HEK293 cells using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 5 μ g/ml.

Immunohistochemistry of B7-H3 in human colon carcinoma tissue using (A) RF16091, (B) RF16092, (C) RF16093, (D) RF16094, (E) RF16095, and (F) control mouse IgG antibody at 2 μ g/ml.

Antibody 1 mg/mL