Item no. |
PRS-PM-4911-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Rat |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
ORAI3 Antibody [2H2G9] : TMEM142C |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Immunology |
Immunogen |
A 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3. |
Applications |
ORAI3 antibody can be used for detection of ORAI3 by Western blot at 1 μ g/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μ g/mL. For immunofluorescence start at 5 μ g/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in rat samples and Immunofluorescence in human and rat samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1466 - Rat Spleen Tissue Lysate |
Predicted Molecular Weight |
Predicted: 32 kDa
Observed: 32 kDa |
Purification |
ORAI3 Monoclonal Antibody is immunoaffinity chromotography purified IgG. |
Clonality |
Monoclonal |
Clone |
2H2G9 |
Isotype |
IgG2a |
Conjugate |
Unconjugated |
Buffer |
ORAI3 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
ORAI3 monoclonal antibody can be stored at -20˚ C, stable for one year. |
Related Products |
Cat. No. 4215P ORAI3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ORAI3 |
Accession # |
Q9BRQ5 |
Protein Gi # |
74732916 |
Ncbi Gene Id # |
93129 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ORAI3 |
Ncbi Official Full Name |
ORAI calcium release-activated calcium modulator 3 |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q9BRQ5 |
Background |
ORAI3 Monoclonal Antibody: Antigen stimulation of immune cells triggers Ca++ entry t hrough Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2. Larger molecular weight bands are sometimes seen in SDS-PAGE; these may represent post-translationally modified ORAI 3. |
Background References 1 |
Lewis RS. Calcium signaling mechanisms in T lymphocytes. Annu. Rev. Immunol. 2001; 19:497-521. |
Background References 2 |
Feske S, Gwack Y, Prakriya M, et al. A mutation in Orai1 causes immune deficiency by abrogating CRAC channel function. Nature 2006; 441:179-85. |
Background References 3 |
Soboloff J, Spassova MA, Dziadek MA, et al. Calcium signals mediated by STIM and Orai proteins - a new paradigm in inter-organelle communication. Biochim. Biophys. Acta. 2006; 1763:1161-8. |
Background References 4 |
Mercer JC, DeHaven WI, Smyth JT, et al. Large store-operated calcium selective currents due to co-expression of Orai1 or Orai2 with the intracellular calcium sensor, Stim1. J. Biol. Chem. 2006; 281:24979-90. |
1st Image Caption |
Western blot analysis of ORAI3 in rat spleen lysate with ORAI3 antibody at 1 μ g/mL. |
2nd Image Caption |
Immunohistochemistry of ORAI3 in rat spleen tissue with ORAI3 antibody at 2.5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of ORAI3 in rat spleen tissue with ORAI3 antibody at 5 μ g/mL. |
4th Image Caption |
Immunofluorescence of ORAI3 in human spleen tissue with ORAI3 antibody at 5 μ g/ml.
Green: ORAI3 antibody (PM-4911) Red: Phylloidin staining Blue: DAPI staining |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.