Item no. |
PRS-PM-4581-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Monoclonal |
Format |
Liquid |
Applications |
WB, ELISA |
Specific against |
Human, Mouse, Rat |
Host |
Mouse |
ECLASS 10.1 |
42030590 |
ECLASS 11.0 |
42030590 |
UNSPSC |
12352203 |
Alias |
EndoG Antibody [7F2D7] : Endo G |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Apoptosis |
Immunogen |
Recombinant protein corresponding to amino acids 51 - 140 of human EndoG. |
Applications |
EndoG monoclonal antibody can be used for detection of EndoG by Western blot at 5 - 10 μ g/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. |
Positive Control 1 |
Cat. No. 1211 - HepG2 Cell Lysate |
Positive Control 2 |
Cat. No. 95-103 - EndoG Recombinant Protein |
Purification |
EndoG Monoclonal Antibody is Protein A purified. |
Clonality |
Monoclonal |
Clone |
7F2D7 |
Isotype |
IgG1 |
Conjugate |
Unconjugated |
Buffer |
EndoG Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
EndoG monoclonal antibody can be stored at -20˚ C, stable for one year. |
Related Products |
Cat. No. 95-103 - EndoG Fragment Recombinant Protein |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
ENDOG |
Accession # |
Q14249 |
Protein Gi # |
24638471 |
Ncbi Gene Id # |
2021 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
ENDOG |
Ncbi Official Full Name |
endonuclease G |
Ncbi Organism |
Homo sapiens |
Swissprot # |
Q14249 |
Background |
EndoG Monoclonal Antibody: The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (EndoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD. EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans. EndoG also cleaves DNA in vitro. |
Background References 1 |
Li LY, Luo X, Wang X. Endonuclease G is an apoptotic DNase when released from mitochondria. Nature 2001; 412:95-9. |
Background References 2 |
Parrish J, Li L, Klotz K, et al. Mitochondrial endonuclease G is important for apoptosis in C. elegans. Nature 2001; 412:90-4 |
Background References 3 |
Hengartner MO. Apoptosis. DNA destroyers. Nature 2001; 412:27, 29. |
Background References 4 |
Widlak P, Li LY, Wang X, et al. Action of recombinant human apoptotic endonuclease G on naked DNA and chromatin substrates: cooperation with exonuclease and DNase I. J. Biol. Chem. 2001; 276:48404-9. |
1st Image Caption |
Western blot analysis of EndoG expression in HepG2 cell lysate with EndoG antibody at (A) 5 and (B) 10 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.