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S1PR2 Antibody

S1PR2 Antibody

Item no.	PRS-8229-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Sphingosine 1-phosphate receptor 2, S1P2, AGR16, EDG-5, EDG5, Gpcr13, H218, LPB2
Available
Shipping	blue ice or RT
By Research Area	Stem Cell, Signal Transduction, Immunology
Immunogen	S1PR2 antibody was raised against an 16 amino acid peptide near the carboxy terminus of human S1PR2. The immunogen is located within amino acids 270 - 320 of S1PR2.
Applications	S1PR2 antibody can be used for detection of S1PR2 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for immunohistochemistry at 10 μ g/ml. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	S1PR2 antibody is human, mouse and rat reactive.
Positive Control 1	Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight	Predicted: 39 kDa Observed: 50 kDa
Mw Explanation	multiple transmembrane protein, also multiple other S1PR2 antibodies also show a ~50kD band
Purification	S1PR2 antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	S1PR2 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	S1PR2 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 8229P – S1PR2 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	S1PR2
Accession #	NP_004221
Protein Gi #	134244587
Ncbi Gene Id #	9294
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	S1PR2
Ncbi Official Full Name	sphingosine-1-phosphate receptor 2
Ncbi Organism	homo sapiens
Swissprot #	O95136
Background	The Sphingosine 1-phosphate receptor 2 (S1PR2) protein was initially identified as G-protein coupled receptor thought to be involved in development (1) and was found to mediate sphingosine 1-phosphate (SPP)-induced cell rounding and neurite retraction (2). S1PR2 and the related protein S1PR3 (also known as EDG3) are also thought to mediate the SPP-induced cell proliferation, survival, and related signaling events (3). S1PR2 has also been found to be key regulator in acute vascular inflammation and may be a novel therapeutic target for vascular disorders (4).
Background References 1	MacLennan AJ, Browe CS, Gaskin AA, et al. Cloning and characterization of a putative G-protein coupled receptor potentially involved in development. Mol. Cell Neurosci. 1994; 5:201-9.
Background References 2	Van Brocklyn JR, Tu Z, Edsall LC, et al. Sphingosine 1-phosphate-induced cell rounding and neurite retraction are mediated by the G protein-coupled receptor H218. J. Biol. Chem. 1999; 274:4626-32.
Background References 3	An S, Zheng Y, and Bleu T. Sphingosine 1-phosphate-induced cell proliferation, survival, and related signaling events mediated by G protein-coupled receptors Edg3 and Edg5. J. Biol. Chem. 275:288-96.
Background References 4	Zhang G, Yang L, Kim GS, et al. Critical role of sphingosine-1-phosphate receptor 2 (S1PR2) in acute vascular inflammation. Blood 2013; 122:443-55.
1st Image Caption	Western blot analysis of S1PR2 in HeLa cell lysate with S1PR2 antibody at 1 μ g/ml.
2nd Image Caption	Immunohistochemistry of S1PR2 in mouse brain tissue with S1PR2 antibody at 10 μ g/ml.
3rd Image Caption	Immunofluorescence of S1PR2 in mouse brain tissue with S1PR2 antibody at 20 μ g/ml. Red: S1PR2 Antibody (8229) Blue: DAPI staining

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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