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HES5 Antibody

HES5 Antibody

Item no.	PRS-8055-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Hairy and enhancer of split 5, hes family bHLH transcription factor 5, bHLHb38
Available
Shipping	blue ice or RT
By Research Area	Stem Cell
Immunogen	HES5 antibody was raised against a 19 amino acid peptide near the amino terminus of human HES5. The immunogen is located within the first 50 amino acids of HES5.
Applications	HES5 antibody can be used for detection of HES5 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human and mouse samples and Immunofluorescence in human and mouse samples. All other applications and species not yet tested.
Specificity	HES5 antibody is human, mouse and rat reactive. At least two isoforms of HES5 are known to exist; this antibody will detect both isoforms.
Positive Control 1	Cat. No. 1207 - Raji Cell Lysate
Positive Control 2	Cat. No. 10-901 - Human Spleen Tissue Slide
Predicted Molecular Weight	Predicted: 18 kDa Observed: 17 kDa
Purification	HES5 antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	HES5 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	HES5 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 8055P – HES5 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	HES5
Accession #	NP_001010926
Protein Gi #	58219048
Ncbi Gene Id #	388585
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	HES5
Ncbi Official Full Name	hes family bHLH transcription factor
Ncbi Organism	homo sapiens
Swissprot #	Q5TA89
Background	HES5 is a member of the HES family of transcriptional repressors, which are the mammalian homologs of the Drosophila Hairy and Enhancer of Split and contain basic Helix-loop-helix (bHLH) and Orange domains (1). The HES5 gene is a downstream target of the Notch signaling pathway and its expression is downregulated during human cartilage differentiation (2). HES5 can directly repress transcription of the E3 ligase FBW7-beta, creating a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions (3).
Background References 1	Katoh M and Katoh M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 2004; 25:529-34.
Background References 2	Karlsson C, Jonsson M, Asp J, et al. Notch and HES5 are regulated during human cartilage differentiation. Cell Tissue Res. 2007; 327:539-51.
Background References 3	Sancho R, Blake SM, Tendeng C, et al. Fbw7 repression by hes5 creates a feedback loop that modulates Notch-mediated intestinal and neural stem cell fate decisions. PLoS Biol. 2013; 11:e1001586.
1st Image Caption	Western blot analysis of HES5 in Raji cell lysate with HES5 antibody at 1 μ g/ml (A) the absence and (B) the presence of blocking peptide.
2nd Image Caption	Immunohistochemistry of HES5 in human spleen tissue with HES5 antibody at 5 μ g/ml.
3rd Image Caption	Immunofluorescence of HES5 in human spleen tissue with HES5 antibody at 20 μ g/ml.
4th Image Caption	Immunohistochemistry of HES5 in mouse brain tissue with HES5 Antibody at 5 μ g/mL.
5th Image Caption	Immunofluorescence of HES5 in mouse brain tissue with HES5 Antibody at 20 μ g/mL. Green: HES5 Antibody (8055) Blue: DAPI staining

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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