« Back
Home /
SKI2W Antibody

SKI2W Antibody

Item no.	PRS-7993-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Neuroscience Neural Lineage Markers Metabolism Diabetes Glucose Homeostasis Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies By Organ Breast Cancer
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Superkiller viralicidic activity 2-like, DDX13, Helicase-like protein, Helicase SKI2W, HLP, SKI2, SKIV2, SKIV2L
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Immunogen	SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W. The immunogen is located within amino acids 60 - 110 of SKI2W.
Applications	SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μ g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL. Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms.
Positive Control 1	Cat. No. 1415 - Mouse Stomach Tissue Lysate
Positive Control 2	Cat. No. 10-801 - Human Stomach Tissue Slide
Predicted Molecular Weight	Predicted: 113, 137 kDa Observed: 135 kDa
Purification	SKI2W antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	SKI2W antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	SKI2W antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7993P – SKI2W Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	SKIV2L
Accession #	NP_008860
Protein Gi #	67782311
Ncbi Gene Id #	6499
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	SKIV2L
Ncbi Official Full Name	SKIV2L superkiller viralicidic activity 2-like (S. cerevisiae)
Ncbi Organism	homo sapiens
Swissprot #	Q15477
Background	SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1, 2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3, 4).
Background References 1	Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6.
Background References 2	Qu X, Yang Z, Zhang S, et al. The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes. Nucleic Acids Res. 1998; 26:4068-77.
Background References 3	Yang Z, Shen L, Dangel AW, et al. Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 1998; 53:338-47.
Background References 4	Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35.
1st Image Caption	Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μ g/ml.
2nd Image Caption	Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μ g/ml.
3rd Image Caption	Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μ g/ml.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

Request product datasheet

Request safety datasheet

Simple Order Order Subscription

Amount: 0.1 mg

available

Delivery expected until 10/4/2024

Express Delivery: Save 11 days

Compare

Add to wishlist

Recommend by mail