Item no. |
PRS-7993-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Superkiller viralicidic activity 2-like, DDX13, Helicase-like protein, Helicase SKI2W, HLP, SKI2, SKIV2, SKIV2L |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Homeostasis |
Immunogen |
SKI2W antibody was raised against a 16 amino acid peptide near the amino terminus of human SKI2W.
The immunogen is located within amino acids 60 - 110 of SKI2W. |
Applications |
SKI2W antibody can be used for detection of SKI2W by Western blot at 1 - 2 μ g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
SKI2W antibody is human, mouse and rat reactive. At least two isoforms of SKI2W are known to exist; this antibody will detect both isoforms. |
Positive Control 1 |
Cat. No. 1415 - Mouse Stomach Tissue Lysate |
Positive Control 2 |
Cat. No. 10-801 - Human Stomach Tissue Slide |
Predicted Molecular Weight |
Predicted: 113, 137 kDa
Observed: 135 kDa |
Purification |
SKI2W antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
SKI2W antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
SKI2W antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7993P – SKI2W Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SKIV2L |
Accession # |
NP_008860 |
Protein Gi # |
67782311 |
Ncbi Gene Id # |
6499 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SKIV2L |
Ncbi Official Full Name |
SKIV2L superkiller viralicidic activity 2-like (S. cerevisiae) |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q15477 |
Background |
SKI2W (SKIV2L, Helicase-like protein), a DEVH-box protein, is a component of the SKI complex which is involved in the regulation of translation and RNA turnover (1, 2). SKI2W is a nucleolar and cytoplasmic protein that has a putative RNA helicase domain (2). The potential roles of Ski2W on the clearance of degraded nuclear and cytoplasmic RNA raised their possibilities as susceptibility genes of systemic lupus erythematosus (3, 4). |
Background References 1 |
Dangel AW, Shen L, Mendoza AR, et al. Human helicase gene SKI2W in the HLA class III region exhibits striking structural similarities to the yeast antiviral gene SKI2 and to the human gene KIAA0052: emergence of a new gene family. Nucleic Acids Res. 1995; 23:2120-6. |
Background References 2 |
Qu X, Yang Z, Zhang S, et al. The human DEVH-box protein Ski2w from the HLA is localized in nucleoli and ribosomes. Nucleic Acids Res. 1998; 26:4068-77. |
Background References 3 |
Yang Z, Shen L, Dangel AW, et al. Four ubiquitously expressed genes, RD (D6S45)-SKI2W (SKIV2L)-DOM3Z-RP1 (D6S60E), are present between complement component genes factor B and C4 in the class III region of the HLA. Genomics 1998; 53:338-47. |
Background References 4 |
Yang Z, Qu X, and Yu CY. Features of the two gene pairs RD-SKI2W and DOM3Z-RP1 located between complement component genes factor B and C4 at the MHC class III region. Front. Biosci. 2001; 6:D927-35. |
1st Image Caption |
Western blot analysis of SKI2W in mouse stomach tissue lysate with SKI2W antibody at 1 μ g/ml. |
2nd Image Caption |
Immunohistochemistry of SKI2W in human stomach tissue with SKI2W antibody at 5 μ g/ml. |
3rd Image Caption |
Immunofluorescence of SKI2W in human stomach tissue with SKI2W antibody at 20 μ g/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.