Item no. |
PRS-7973-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
Kin of irregular chiasm-like protein 3, Kin of IRRE-like protein 3, MRD4, KIRRE, NEPH2, PRO4502, Nephrin-like protein 2 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Neuroscience, Stem Cell |
Immunogen |
KIRREL3 antibody was raised against a 19 amino acid peptide near the amino terminus of human KIRREL3.
The immunogen is located within amino acids 20 - 70 of KIRREL3. |
Applications |
KIRREL3 antibody can be used for detection of KIRREL3 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Specificity |
KIRREL3 antibody is human, mouse and rat reactive. At least two isoforms are known to exist; this antibody will detect both isoforms. KIRREL3 antibody is predicted to not cross-react with other members of the KIRREL protein family. |
Positive Control 1 |
Cat. No. 1405 - Mouse Kidney Tissue Lysate |
Predicted Molecular Weight |
Predicted: 64, 84 kDa
Observed: 60 kDa |
Purification |
KIRREL3 antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
KIRREL3 antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
KIRREL3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7973P – KIRREL3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
KIRREL3 |
Accession # |
NP_115920 |
Protein Gi # |
26006461 |
Ncbi Gene Id # |
84623 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
KIRREL3 |
Ncbi Official Full Name |
kin of IRRE like 3 (Drosophila) |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q8IZU9 |
Background |
KIRREL3, also known as nephrin-like protein 2, is a type I transmembrane protein belonging to a family of three podocin interacting proteins and the immunoglobulin superfamily (1). KIRREL3 is involved in the regulation of both glomerular and neural development (2), and more specifically, the nucleogenesis of the pontine nuclei in the developing hindbrain (3). KIRREL3 has also been shown to interact with the synaptic scaffold protein calmodulin-associated serine/threonine kinase (CASK) in neuronal cells (4). |
Background References 1 |
Sellin L, Huber TB, Gerke P, et al. NEPH1 defines a novel family of podocin interacting proteins. FASEB J. 2003; 17:115-7. |
Background References 2 |
Neumann-Haefelin E, Kramer-Zucker A, Slanchev K, et al. A model organism approach: defining the role of Neph proteins as regulators of neuron and kidney morphogenesis. Hum. Mol. Genet. 2010; 19:2347-59. |
Background References 3 |
Nishida K, Nakayama K, Yoshimura S, et al. Role of Neph2 in pontine nuclei formation in the developing hindbrain. Mol. Cell Neurosci. 2011; 46:662-70. |
Background References 4 |
Mizuhara E, Minaki Y, Nakatani T, et al. Purkinje cells originate from cerebellar ventricular zone progenitors positive for Neph3 and E-cadherin. Dev. Biol. 2010; 338:202-14. |
1st Image Caption |
Western blot analysis of KIRREL3 in mouse kidney tissue lysate with KIRREL3 antibody at (A) 1 and (B) 2 μ g/ml. |
2nd Image Caption |
Immunohistochemistry of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 5 μ g/ml. |
3rd Image Caption |
Immunofluorescence of KIRREL3 in mouse kidney tissue with KIRREL3 antibody at 20 μ g/ml. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.