Item no. |
PRS-79-854-0.1mg |
Manufacturer |
ProSci
|
Amount |
0.1 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IHC |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
MAP2K3, MAPKK 3, MEK3, MP2K3, PRKMK3 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Phospho-Specific |
Immunogen |
MAP2K3 (phospho Thr222) antibody was raised against a peptide sequence around phosphorylation site of threonine 222 (A-K-T (p)-M-D) derived from Human MAP2K3 . |
Applications |
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100 |
Specificity |
The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222. |
Predicted Molecular Weight |
39 kDa |
Purification |
Antibodies were purified by affinity-chromatography using epitope-specific peptide. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Concentration |
1 mg/mL |
Storage Conditions |
Store antibody at -20˚ C for up to one year. |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
Phospho-Specific |
Ncbi Official Symbol |
MAP2K3 |
Accession # |
NP_659731.1 |
Protein Gi # |
24638466 |
Ncbi Gene Id # |
5606 |
Ncbi Official Symbol |
MAP2K3 |
Ncbi Official Full Name |
mitogen-activated protein kinase kinase 3 |
Ncbi Organism |
Homo sapiens |
Swissprot # |
P46734 |
Background |
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. |
Background References 1 |
Derijard B., Science 267:682-685(1995). |
Background References 2 |
Moriguchi T., J. Biol. Chem. 271:26981-26988(1996). |
Background References 3 |
The MGC Project Team; Genome Res. 14:2121-2127(2004). |
1st Image Caption |
Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is treated with the antigen-specific peptide. |
2nd Image Caption |
Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody (left) or the same antibody preincubated with blocking peptide (right). |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.