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MAP2K3 (phospho Thr222) Antibody

MAP2K3 (phospho Thr222) Antibody

Item no.	PRS-79-854-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Neuroscience Neural Lineage Markers Metabolism Diabetes Cholesterol & Lipid Metabolism Regulation of Appetite Antibodies Immunohistochemistry Western Blot Primary Antibodies Glial Cell Markers
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	MAP2K3, MAPKK 3, MEK3, MP2K3, PRKMK3
Available
Shipping	blue ice or RT
By Research Area	Phospho-Specific
Immunogen	MAP2K3 (phospho Thr222) antibody was raised against a peptide sequence around phosphorylation site of threonine 222 (A-K-T (p)-M-D) derived from Human MAP2K3 .
Applications	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
Specificity	The antibody detects endogenous levels of MAP2K3 only when phosphorylated at threonine 222.
Predicted Molecular Weight	39 kDa
Purification	Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/mL
Storage Conditions	Store antibody at -20˚ C for up to one year.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	Phospho-Specific
Ncbi Official Symbol	MAP2K3
Accession #	NP_659731.1
Protein Gi #	24638466
Ncbi Gene Id #	5606
Ncbi Official Symbol	MAP2K3
Ncbi Official Full Name	mitogen-activated protein kinase kinase 3
Ncbi Organism	Homo sapiens
Swissprot #	P46734
Background	The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells.
Background References 1	Derijard B., Science 267:682-685(1995).
Background References 2	Moriguchi T., J. Biol. Chem. 271:26981-26988(1996).
Background References 3	The MGC Project Team; Genome Res. 14:2121-2127(2004).
1st Image Caption	Western blot analysis of extracts from Jurkat cells treated with serum using MAP2K3 (Phospho-Thr222) Antibody. The lane on the right is treated with the antigen-specific peptide.
2nd Image Caption	Immunohistochemical analysis of paraffin-embedded human brain tissue using MAP2K3 (Phospho-Thr222) antibody (left) or the same antibody preincubated with blocking peptide (right).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.1 mg

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