PTEN (phospho Ser380 / Thr382 / Thr383) Antibody

blue ice or RT

PTEN (Phospho-Ser380/Thr382/Thr383) antibody was raised against a peptide sequence around phosphorylation site of threonine 380/382/383 (R-Y-S (p) -D-T (p) -T (p) -D-S) derived from Human PTEN.

This antibody detects endogenous level of PTEN only when phosphorylated at serine 380 and threonine 382/383.

Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Unconjugated

1 mg/mL

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

PTEN

73765544

PTEN

Homo sapiens

Tumor suppressor. Acts as a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Also acts as a lipid phosphatase, removing the phosphate in the D3 position of the inositol ring from phosphatidylinositol 3, 4, 5-trisphosphate, phosphatidylinositol 3, 4-diphosphate, phosphatidylinositol 3-phosphate and inositol 1, 3, 4, 5-tetrakisphosphate with order of substrate preference in vitro PtdIns(3, 4, 5)P3 > PtdIns(3, 4)P2 > PtdIns3P > Ins(1, 3, 4, 5)P4. The lipid phosphatase activity is critical for its tumor suppressor function. Antagonizes the PI3K-AKT/PKB signaling pathway by dephosphorylating phosphoinositides and thereby modulating cell cycle progression and cell survival. The unphosphorylated form cooperates with AIP1 to suppress AKT1 activation. Dephosphorylates tyrosine-phosphorylated focal adhesion kinase and inhibits cell migration and integrin-mediated cell spreading and focal adhesion formation. May be a negative regulator of insulin signaling and glucose metabolism in adipose tissue.

Western blot analysis of lysed extracts from HeLa cells using PTEN (Phospho-Ser380/Thr382/Thr383).