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MEK 2 (phospho Thr394) Antibody

MEK 2 (phospho Thr394) Antibody

Item no.	PRS-79-106-0.1mg
Manufacturer	ProSci
Amount	0.1 mg
Category	Oncology Neuroscience Neural Signaling Antibodies By Cell Type Blastoma Carcinoma Glioma Hematology Lymphoma Myeloma Melanoma Sarcoma Immunohistochemistry Western Blot Immunofluorescence Primary Antibodies
Type	Antibody Primary
Format	Liquid
Applications	WB, IF, IHC
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	CFC4, MEK2, MKK2, MAPKK2, PRKMK2, SRC1, ERK activator kinase 2, MAP kinase kinase 2, MAP2K2, MAPK/ERK kinase 2, MAPKK 2
Available
Shipping	blue ice or RT
By Research Area	Phospho-Specific
Immunogen	MEK-2 (Phospho-Thr394) antibody was raised against a peptide sequence around phosphorylation site of threonine 394 (P-G-T (p) -P-T) derived from Human MEK-2.
Applications	Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity	This antibody detects endogenous level of MEK2 only when phosphorylated at threonine 394.
Predicted Molecular Weight	44 kDa
Purification	Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality	Polyclonal
Conjugate	Unconjugated
Buffer	Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration	1 mg/mL
Storage Conditions	Store antibody at -20˚ C for up to one year.
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	Phospho-Specific
Ncbi Official Symbol	MAP2K2
Accession #	NP_109587.1
Protein Gi #	13489054
Ncbi Gene Id #	5605
Ncbi Official Symbol	MAP2K2
Ncbi Official Full Name	mitogen-activated protein kinase kinase 2
Ncbi Organism	Homo sapiens
Swissprot #	P36507
Background	Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases.
Background References 1	Crews C M, et al. (1992) Science. 258:478-480. Alessi D R, et al. (1994) EMBO J. 13:1610-1619. Rosen L B, et al. (1994) Neuron. 12:1207-1221. Cowley S, et al. (1994) Cell. 77:841-852.
1st Image Caption	Western blot analysis of lysed extracts from HepG2 and HeLa cells untreated or treated with UV using MEK-2 (Phospho-Thr394).
2nd Image Caption	Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK-2 (Phospho-Thr394).Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using MEK-2 (Phospho-Thr394).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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