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PDCL3 Antibody

PDCL3 Antibody

Item no.	PRS-7889-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Antibodies Immunohistochemistry Western Blot ELISA Immunofluorescence Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ELISA, IHC-P
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	PDCL3 Antibody: VIAF, PHLP3, VIAF1, HTPHLP, PHLP2A, PhLP2A, Phosducin-like protein 3, VIAF-1
Available
Shipping	blue ice or RT
By Research Area	Signal Transduction
Immunogen	PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3. The immunogen is located within the last 50 amino acids of PDCL3.
Applications	PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry at 5 μ g/mL. For Immunoflorescence start at 20 μ g/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity	PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family.
Positive Control 1	Cat. No. 1303 - Human Brain Tissue Lysate
Predicted Molecular Weight	Predicted: 26 kDa Observed: 25 kDa
Purification	PDCL3 antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	PDCL3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	PDCL3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7889P – PDCL3 Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	PDCL3
Accession #	NP_076970
Protein Gi #	13129044
Ncbi Gene Id #	79031
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	PDCL3
Ncbi Official Full Name	phosducin-like 3
Ncbi Organism	homo sapiens
Swissprot #	Q9H2J4
Background	Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1, 2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5).
Background References 1	Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5.
Background References 2	Ruiz-Gomez A, Humrich J, Murga C, et al. Phosphorylation of phosducin and phosducinlike protein by G protein-coupled receptor kinase 2. J. Biol. Chem. 2000; 275:29724-30.
Background References 3	Lou X, Bao R, Zhou CZ, et al. Structure of the thioredoxin-fold domain of human phosducin-like protein 2. Acta Crystallographica 2009; F65:67-70.
Background References 4	Stirling PC, Srayko M, Takhar KS, et al. Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeltal protein function and cell cycle progrssion. Mol. Biol. Cell 2007; 18:2336-45.
1st Image Caption	Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μ g/ml.
2nd Image Caption	Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μ g/mL.
3rd Image Caption	Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μ g/mL.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 0.02 mg

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Delivery expected until 9/6/2024