Item no. |
PRS-7889-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human, Mouse, Rat |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
PDCL3 Antibody: VIAF, PHLP3, VIAF1, HTPHLP, PHLP2A, PhLP2A, Phosducin-like protein 3, VIAF-1 |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Signal Transduction |
Immunogen |
PDCL3 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human PDCL3.
The immunogen is located within the last 50 amino acids of PDCL3. |
Applications |
PDCL3 antibody can be used for detection of PDCL by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry at 5 μ g/mL. For Immunoflorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. |
Specificity |
PDCL3 antibody is human, mouse and rat reactive. PDCL3 antibody is predicted to not cross-react with other members of the PDCL protein family. |
Positive Control 1 |
Cat. No. 1303 - Human Brain Tissue Lysate |
Predicted Molecular Weight |
Predicted: 26 kDa
Observed: 25 kDa |
Purification |
PDCL3 antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
PDCL3 antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
PDCL3 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7889P – PDCL3 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
PDCL3 |
Accession # |
NP_076970 |
Protein Gi # |
13129044 |
Ncbi Gene Id # |
79031 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
PDCL3 |
Ncbi Official Full Name |
phosducin-like 3 |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q9H2J4 |
Background |
Phosducin-like proteins (PhLPs) are a conserved family of proteins with thioredoxin-like domains that were initially identified as modulators of G protein signaling (1, 2). PDCL3 is highly homologous to PDCL and shares an N-terminal helix domain and a C-terminal thioredoxin-fold (Trx-fold) domain (3). Along with the related protein PDCL2, PDCL3 interacts with the chaperonin CCT and modulates CCT-mediated actin and tubulin folding (4). Modulation of PDCL3 levels by MAPK phosphorylation and RhoA-dependent changes also promote cytoskeletal remodeling (5). |
Background References 1 |
Miles MF, Barhite S, Sganga M, et al. Phosducin-like protein: an ethanol-responsive potential modulator of guanine nucleotide-binding protein function. Proc. Natl. Acad. Sci. USA 1993; 90:10831-5. |
Background References 2 |
Ruiz-Gomez A, Humrich J, Murga C, et al. Phosphorylation of phosducin and phosducinlike protein by G protein-coupled receptor kinase 2. J. Biol. Chem. 2000; 275:29724-30. |
Background References 3 |
Lou X, Bao R, Zhou CZ, et al. Structure of the thioredoxin-fold domain of human phosducin-like protein 2. Acta Crystallographica 2009; F65:67-70. |
Background References 4 |
Stirling PC, Srayko M, Takhar KS, et al. Functional interaction between phosducin-like protein 2 and cytosolic chaperonin is essential for cytoskeltal protein function and cell cycle progrssion. Mol. Biol. Cell 2007; 18:2336-45. |
1st Image Caption |
Western blot analysis of PDCL3 in human brain tissue lysate with PDCL3 antibody at 1 μ g/ml. |
2nd Image Caption |
Immunohistochemistry of PDCL3 in mouse lver tissue with PDCL3 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of PDCL3 in mouse liver tissue with PDCL3 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.