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Vinculin Antibody

Vinculin Antibody

Item no.	PRS-7809-0.02mg
Manufacturer	ProSci
Amount	0.02 mg
Category	Oncology Antibodies Western Blot ELISA Immunofluorescence Immunocytochemistry Primary Antibodies
Type	Antibody Polyclonal
Format	Liquid
Applications	WB, IF, ICC, ELISA
Specific against	Human, Mouse, Rat
Host	Rabbit
ECLASS 10.1	32160702
ECLASS 11.0	32160702
UNSPSC	12352203
Alias	Vinculin Antibody: MV, MVCL, CMD1W, CMH15, HEL114, Vinculin, Metavinculin, MV
Available
Shipping	blue ice or RT
By Research Area	Homeostasis
Homology	Predicted species reactivity based on immunogen sequence: Pig: (100%)
Immunogen	Anti-Vinculin antibody (7809) was raised against a peptide corresponding to 23 amino acids near the center of human Vinculin. <
Applications	WB: 0.5-2 μ g/mL; ICC: 5 μ g/mL; IF: 20 μ g/mL. Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity	Vinculin antibody is human, mouse and rat reactive. At least three isoforms of Vinculin are known to exist.
Positive Control 1	Cat. No. 1202 - A431 Cell Lysate
Positive Control 2	Cat. No. 1201 - HeLa Cell Lysate
Positive Control 3	Cat. No. 1282 - 3T3/NIH Cell Lysate
Predicted Molecular Weight	Predicted: 124kD Observed: 124kD
Validation	Independent Antibody Validation in Cell lines (Figure 2) shows similar Vinculin expression profile in human, mouse and rat cell lines detected by three independent anti-Vinculin antibodies that recognize different epitopes, 7489 against C-terminus domain, 7807 against N-terminus domain and 7809 against central domain. Vinculin proteins are detected in the most tested cell lines at different expression levels by the three independent antibodies.
Isoforms	Human Vinculin has 3 isoforms, including isoform 2 (1134aa, 124kD), isoform 1 (1066aa, 117kD) and isoform 3 (222aa, 25kD). Mouse Vinculin has one isoform (1066aa, 117kD) and Rat Vinculin also has one isoform (1066aa, 117kD). 7809 can detect human, mouse and rat.
Purification	Vinculin antibody is affinity chromatography purified via peptide column.
Clonality	Polyclonal
Isotype	IgG
Conjugate	Unconjugated
Buffer	Vinculin antibody is supplied in PBS containing 0.02% sodium azide.
Concentration	1 mg/mL
Storage Conditions	Vinculin antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year.
Related Products	Cat. No. 7809P – Vinculin Peptide
Disclaimer	Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Modifications	None
Ncbi Official Symbol	VCL
Accession #	NP_003364
Protein Gi #	4507877
Ncbi Gene Id #	7414
User Note	Optimal dilutions for each application to be determined by the researcher.
Ncbi Official Symbol	VCL
Ncbi Official Full Name	vinculin
Ncbi Organism	homo sapiens
Swissprot #	P18206
Background	Vinculin is a cytoskeletal protein that plays an important role in the regulation of focal adhesions and embryonic development (1). Three structural vinculin domains include an amino-terminal head, a short flexible proline-rich region and a carboxy-terminal tail (2). Expression of vinculin were shown to be affected by the level of actin expression (2, 3). Vinculin deficiencies are associated with a decrease in cell adhesion and an increase in cell motility, suggesting a possible role in metastatic growth (4). Defects in VCL are the cause of cardiomyopathy dilated type 1W (CMD1W) (5).
Background References 1	Burridge et al. Annu. Rev. Cell Biol.1988; 4:487-525.
Background References 2	Gilmore et al. J. Cell Sci. 1992; 103:719-31.
Background References 3	Deakin et al. PLoS One 2012; 7:e37990.
Background References 4	Goldmann et al. Cell Biol. Int. 2013; Feb 1.
Citation 1	Wang et al. AMPK is involved in the differential neonatal performance of chicks hatching at different time. Gen Comp Endocrinol. 2016; 228:53-59. PMID: 26873631
1st Image Caption	Figure 1 Independent Antibody Validation (IAV) via Protein Expression Profile in Human, Mouse and Rat Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: Vinculin 7489 (2 μ g/mL), Vinculin 7807 (1 μ g/mL), Vinculin 7809 (1 µg/mL), and beta-actin 3779 (1.5 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
2nd Image Caption	Figure 2 Western Blot Validation in Human, Mouse and Rat Cell Lines Loading: 15 μ g of lysates per lane.Antibodies: Vinculin 7809 (1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
3rd Image Caption	Figure 3 Western Blot Validation in PC3 Cell Lysate Loading: 15 μ g of lysates per lane.Antibodies: Vinculin 7809 (Lane 1: 0.5 μ g/mL and Lane 2: 1 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
4th Image Caption	Figure 4 Immunocytochemistry Validation of Vinculin in Jurkat Cells Immunocytochemical analysis of Jurkat cells using anti-Vinculin antibody (7809) at 5 μ g/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
5th Image Caption	Figure 5 Immunofluorescence Validation of Vinculin in Jurkat Cells Immunofluorescent analysis of 4% paraformaldehyde-fixed Jurkat cells labeling Vinculin with 7809 at 20 μ g/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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