TRIM21 Antibody

blue ice or RT

Predicted species reactivity based on immunogen sequence: Bovine (73%), Mouse (69%) and Rat (69%).

WB: 0.5-2 μ g/mL; IHC: 5 μ g/mL; IF: 20 μ g/mL.

Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunofluorescence in human samples. All other applications and species not yet tested.

Cat. No. 1302 - Human Lung Tissue Lysate

Cat. No. 1201 - HeLa Cell Lysate

Predicted: 54kD

Observed: 54 kD

Human TRIM21 has 2 isoforms, including isoform 1 (475aa, 54kD) and isoform 2 (398aa, 45kD). Mouse TRIM21 has one isoform (470aa, 54kD) and Rat TRIM21 also has one isoform (471aa, 54kD). 7729 can detect human and possibly mouse and rat.

Polyclonal

Unconjugated

1 mg/mL

Cat. No. 7729P – TRIM21 Peptide

None

NP_003132

6737

TRIM21

homo sapiens

TRIM21, a member of the tripartite motif (TRIM) family, is a part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus (1). RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus (2). TRIM21 also acts as a cytosolic antibody receptor; detection of intracellular antibodies activate immune signaling by stimulating the transcription factor pathways of NF-kappaB, AP-1, IRF3, IRF5, and IRF7, resulting in the production of proinflammatory cytokines and the induction of an antiviral state (3).

Frank et al. Biochem. J. 1995; 305:359-62.

Figure 1 Western Blot Validation in Human Lung Tissue Lysate in (Lane 1) the absence and (Lane 2) the presence of blocking peptide
Loading: 15 μ g of lysates per lane.Antibodies: TRIM21, 7729 (0.5 μ g/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Figure 3 Immunohistochemistry Validation of TRIM21 in Human Small Intestine Tissue
Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TRIM21 antibody (7729) at 5 μ g/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚ C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.