Item no. |
PRS-7701-0.02mg |
Manufacturer |
ProSci
|
Amount |
0.02 mg |
Category |
|
Type |
Antibody Polyclonal |
Format |
Liquid |
Applications |
WB, IF, ELISA, IHC-P |
Specific against |
Human |
Host |
Rabbit |
ECLASS 10.1 |
32160702 |
ECLASS 11.0 |
32160702 |
UNSPSC |
12352203 |
Alias |
SUZ12 Antibody: CHET9, JJAZ1, CHET9, KIAA0160, Polycomb protein SUZ12, Chromatin precipitated E2F target 9 protein, ChET 9 protein |
Available |
|
Shipping |
blue ice or RT |
By Research Area |
Stem Cell |
Homology |
Predicted species reactivity based on immunogen sequence: Mouse: (79%) |
Immunogen |
SUZ12 antibody was raised against a 19 amino acid peptide near the center of human SUZ12.
The immunogen is located within amino acids 400 - 450 of SUZ12. |
Applications |
SUZ12 antibody can be used for detection of SUZ12 by Western blot at 1 - 2 μ g/ml. Antibody can also be used for Immunohistochemistry starting at 5 μ g/mL. For immunofluorescence start at 20 μ g/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. |
Specificity |
SUZ12 antibody is human specific. |
Positive Control 1 |
Cat. No. 1304 - Human Liver Tissue Lysate |
Positive Control 2 |
Cat. No. 10-101 - Human Lung Tissue Slide |
Predicted Molecular Weight |
Predicted: 81 kDa
Observed: 96 kDa |
Purification |
SUZ12 antibody is affinity chromatography purified via peptide column. |
Clonality |
Polyclonal |
Isotype |
IgG |
Conjugate |
Unconjugated |
Buffer |
SUZ12 antibody is supplied in PBS containing 0.02% sodium azide. |
Concentration |
1 mg/mL |
Storage Conditions |
SUZ12 antibody can be stored at 4˚ C for three months and -20˚ C, stable for up to one year. |
Related Products |
Cat. No. 7701P – SUZ12 Peptide |
Disclaimer |
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only. |
Modifications |
None |
Ncbi Official Symbol |
SUZ12 |
Accession # |
NP_056170 |
Protein Gi # |
197333809 |
Ncbi Gene Id # |
23512 |
User Note |
Optimal dilutions for each application to be determined by the researcher. |
Ncbi Official Symbol |
SUZ12 |
Ncbi Official Full Name |
SUZ12 polycomb repressive complex 2 subunit |
Ncbi Organism |
homo sapiens |
Swissprot # |
Q15022 |
Background |
The Polycomb group (PcG) genes contribute to the maintenance of cell identity, cell cycle regulation and oncogenesis (1). SUZ12 (suppressor of zeste 12) is a 90 - 95 kDa member of the polycomb group of transcriptional regulators (2). It is part of a transcription repression complex termed PRC2 (Polycomb repressive complex 2) that methylates histones in the nucleus, resulting in homeotic (pattern-inducing) gene silencing. It contains one C2H2-type zinc finger region and a C-terminal VEFS-box (3, 4). SUZ12 is overexpressed in breast and colon cancer. SUZ12 may be a cause of endometrial stromal tumors (5). |
Background References 1 |
Kirmizis A, Bartley SM, Kuzmichev A, et al. Silencing of human polycomb target genes is associated with methylation of Histone H3 Lys 27. Genes Dev. 2004; 18:1592-605. |
Background References 2 |
Pasini D, Bracken AP, Hansen JB, et al. The polycomb group protein Suz12 is required for embryonic stem cell differentiation. Mol. Cell. Biol. 2007; 27:3769-79. |
Background References 3 |
Cao R and Zhang Y. SUZ12 is required for both the Histone methyltransferase activity and the silencing function of the EED-EZH2 complex. Mol. Cell 2004; 15:57-67. |
Background References 4 |
Pasini D, Bracken AP, Jensen MR, et al. SUZ12 is essential for mouse development and for EZH2 Histone methyltransferase activity. EMBO J. 2004; 23:4061-71. |
1st Image Caption |
Western blot analysis of SUZ12 in human liver tissue lysate with SUZ12 antibody at 1 μ g/ml. |
2nd Image Caption |
Immunohistochemistry of SUZ12 in human lung tissue with SUZ12 antibody at 5 μ g/mL. |
3rd Image Caption |
Immunofluorescence of SUZ12 in human lung tissue with SUZ12 antibody at 20 μ g/mL. |
Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.
All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.